Adding L-3-(2-naphthyl)alanine to the genetic code of E-coli
Adding L-3-(2-naphthyl)alanine to the genetic code of E-coli
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DOI：
10.1021/ja012307j
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发表时间：
2002-03-06
影响因子：
15
通讯作者：
Schultz, PG
中科院分区：
文献类型：
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作者：
Wang, L;Brock, A;Schultz, PG
1 -3-（2-萘基）丙氨酸是一种非天然氨基酸，已被大肠杆菌定点掺入蛋白质中。进化出一种正交氨酰-tRNA合成酶，其独特地将非天然氨基酸氨酰化到正交琥珀抑制tRNA上，其响应于琥珀无义密码子以大于99%的翻译保真度递送酰化氨基酸。这一结果，连同体内位点特异性掺入ofO-甲基-L-酪氨酸先前报道，证明这种方法可能适用于主机的氨基酸。将遗传密码扩展到包括20种常见氨基酸以外的氨基酸，将提供一个更好地理解和可能增强蛋白质（也许还有生物体）功能的机会。
An unnatural amino acid,l-3-(2-naphthyl)alanine, has been site-specifically incorporated into proteins inEscherichia coli. An orthogonal aminoacyl-tRNA synthetase was evolved that uniquely aminoacylates the unnatural amino acid onto an orthogonal amber suppressor tRNA, which delivers the acylated amino acid in response to an amber nonsense codon with translational fidelity greater than 99%. This result, together with the in vivo site-specific incorporation ofO-methyl-l-tyrosine reported previously, demonstrate that this methodology may be applicable to a host of amino acids. The expansion of the genetic code to include amino acids beyond the common 20 would provide an opportunity to better understand and possibly enhance protein (and perhaps organismal) function.