DNA fingerprinting of the NCI-60 cell line panel.
DNA fingerprinting of the NCI-60 cell line panel.
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DOI：
10.1158/1535-7163.mct-08-0921
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发表时间：
2009-04
影响因子：
5.7
通讯作者：
Weinstein JN
中科院分区：
文献类型：
--
作者：
Lorenzi PL;Reinhold WC;Varma S;Hutchinson AA;Pommier Y;Chanock SJ;Weinstein JN
美国国家癌症研究所的NCI-60细胞系面板，现有的最广泛表征的细胞集和公共资源，经常被用作药物发现的筛选工具。由于世界各地的许多实验室依赖于NCI-60细胞的数据，因此确认其遗传身份是验证其结果的重要步骤。鉴于细胞系污染或错误鉴定的后果，质量控制措施应常规包括DNA指纹图谱。因此，我们使用标准的DNA微卫星短串联重复序列来分析NCI-60，并在此提供所得的DNA指纹作为参考。与之前的报道一致，指纹表明几种NCI-60细胞系具有共同的起源：黑素瘤细胞系MDA-MB-435、MDA-N和M14;中枢神经系统细胞系U251和SNB-19;卵巢细胞系OVCAR-8和OVCAR-8/ADR（也称为NCI/ADR）;以及前列腺细胞系DU-145、DU-145（ATCC）和RC 0.1。这些细胞系还证明，将两个指纹连接到同一来源的能力不受稳定转染或多药耐药性发展的影响。正如预期的那样，DNA指纹无法区分不同的组织来源。指纹主要用作条形码。
The National Cancer Institute’s NCI-60 cell line panel, the most extensively characterized set of cells in existence and a public resource, is frequently used as a screening tool for drug discovery. Since many laboratories around the world rely on data from the NCI-60 cells, confirmation of their genetic identities represents an essential step in validating results from them. Given the consequences of cell line contamination or misidentification, quality control measures should routinely include DNA fingerprinting. We have, therefore, used standard DNA microsatellite short tandem repeats to profile the NCI-60, and the resulting DNA fingerprints are provided here as a reference. Consistent with previous reports, the fingerprints suggest that several NCI-60 lines have common origins: the melanoma lines MDA-MB-435, MDA-N, and M14; the central nervous system lines U251 and SNB-19; the ovarian lines OVCAR-8 and OVCAR-8/ADR (also called NCI/ADR); and the prostate lines DU-145, DU-145 (ATCC), and RC0.1. Those lines also demonstrate that the ability to connect two fingerprints to the same origin is not affected by stable transfection or by the development of multidrug resistance. As expected, DNA fingerprints were not able to distinguish different tissues-of-origin. The fingerprints serve principally as a barcodes.