Prognostic Relevance and Function of MSX2 in Colorectal Cancer.
Prognostic Relevance and Function of MSX2 in Colorectal Cancer.
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DOI：
10.1155/2017/3827037
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发表时间：
2017
影响因子：
4.3
通讯作者：
Ma J
中科院分区：
文献类型：
--
作者：
Liu J;An H;Yuan W;Feng Q;Chen L;Ma J
结直肠癌合并糖尿病患者总死亡率高。MSX 2的高表达与糖尿病的发生有关。目前关于MSX 2在结直肠癌中的作用及其临床意义的研究报道较少。本研究旨在探讨MSX 2在结直肠癌组织中的表达及其与临床的关系，并探讨MSX 2在结直肠癌发生发展中的可能作用机制。与癌旁组织相比，MSX 2在mRNA和蛋白水平上的表达均显著增高（P < 0.01）。Kaplan-Meier生存分析显示MSX 2 mRNA高表达与生存时间短相关（P = 0.013）。卡方检验显示MSX 2表达与肿瘤大小（P = 0.04）、肿瘤部位（P = 0.025）、临床分期（P < 0.001）、肿瘤浸润（P = 0.003）、淋巴结转移（P = 0.01）和远处转移（P = 0.033）有关。体外实验表明，MSX 2表达的敲低减弱细胞增殖和侵袭，促进细胞周期停滞和凋亡，并灭活Akt磷酸化。总之，MSX 2在结直肠癌的进展中起着至关重要的作用，并可能成为结直肠癌治疗的一个潜在的新的预后因子和治疗靶点。我们的工作可能为探讨MSX 2在糖尿病发病过程中的作用机制提供一定的启示。
Colorectal cancer patients with diabetes had the high risks of total mortality. High expression of MSX2 is related to development of diabetes. There are few reports about the clinical implications and function of MSX2 in colorectal cancer (CRC). The purpose of this study is to investigate the relationship between the expression of MSX2 and clinical relevance and discover the possible mechanism of MSX2 in the development of CRC. Compared with adjacent tissues, the expression of MSX2 was higher in tumor tissues in both mRNA and protein levels (P < 0.01). Kaplan-Meier survival analysis showed that high mRNA expression of MSX2 was associated with short survival time (P = 0.013). Chi-squared test analysis indicated that MSX2 expression was related to tumor size (P = 0.04), tumor locus (P = 0.025), clinical stage (P < 0.001), tumor invasion (P = 0.003), lymphatic metastasis (P = 0.01), and distant metastasis (P = 0.033). In vitro experiments demonstrated that knockdown of MSX2 expression attenuated cell proliferation and invasion, promoted cell cycle arrest and apoptosis, and inactivated Akt phosphorylation. In conclusion, MSX2 played a crucial role in the progression of CRC and may be a potential novel prognostic factor and therapeutic target for CRC therapy. Our work may provide certain enlightenment for investigating the mechanism of MSX2 in the process of diabetes.