Induction of nerve growth factor receptors on cultured human melanocytes.
Induction of nerve growth factor receptors on cultured human melanocytes.
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DOI：
10.1073/pnas.85.14.5282
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发表时间：
1988-07
影响因子：
11.1
通讯作者：
M. Peacocke;M. Yaar;C. Mansur;M. Chao;Barbara A. Gilchrest
中科院分区：
文献类型：
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作者：
M. Peacocke;M. Yaar;C. Mansur;M. Chao;Barbara A. Gilchrest
人类黑素细胞的正常分化和恶性转化涉及一系列复杂的相互作用，在此过程中遗传和环境因素都起作用。目前，对这些过程的调控知之甚少。我们已经诱导了神经生长因子（NGF）受体的表达与佛波醇12-十四烷酸酯13-乙酸培养的人黑素细胞，并与此事件的外观更分化，树突状形态。神经生长因子受体表达的标准包括蛋白质积累和细胞表面免疫荧光染色的单克隆抗体针对人类受体和诱导的信使RNA物种所确定的印迹杂交研究。受体的存在也可以通过UV照射或生长因子剥夺来诱导。神经生长因子受体是诱导培养的人黑素细胞，我们认为，神经生长因子可以调节这种神经嵴衍生细胞在皮肤中的行为。
Normal differentiation and malignant transformation of human melanocytes involve a complex series of interactions during which both genetic and environmental factors play roles. At present, the regulation of these processes is poorly understood. We have induced the expression of nerve growth factor (NGF) receptors on cultured human melanocytes with phorbol 12-tetradecanoate 13-acetate and have correlated this event with the appearance of a more differentiated, dendritic morphology. Criteria for NGF receptor expression included protein accumulation and cell-surface immunofluorescent staining with a monoclonal antibody directed against the human receptor and induction of the messenger RNA species as determined by blot-hybridization studies. The presence of the receptor could also be induced by UV irradiation or growth factor deprivation. The NGF receptor is inducible in cultured human melanocytes, and we suggest that NGF may modulate the behavior of this neural crest-derived cell in the skin.