Kinetic effect of silkworm hemolymph on the delayed host cell death in an insect cell-baculovirus system
Kinetic effect of silkworm hemolymph on the delayed host cell death in an insect cell-baculovirus system
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DOI：
10.1021/bp990093s
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发表时间：
1999-11-01
影响因子：
2.9
通讯作者：
Park, TH
中科院分区：
文献类型：
--
作者：
Rhee, WJ;Kim, EJ;Park, TH
研究了杆状病毒诱导的昆虫细胞死亡过程中蚕血淋巴对宿主细胞活力的动力学影响。病毒感染后宿主细胞的活力对于含有重组基因的杆状病毒DNA的复制和克隆基因的表达非常重要。杆状病毒诱导的昆虫细胞死亡过程可分为延迟期和一级死亡期，分别以延迟时间（t（d））和比死亡率（k（d））为特征。对于培养基中0-10%的蚕血淋巴，较高的浓度导致较长的延迟时间和较低的特定死亡率。添加10%蚕血淋巴后，延迟时间由72 h增加至164 h，比死亡率由13.8×10(-3)降低至6.0×10(-3)h(-1)。此外，宿主细胞活力与DNA片段化相关，DNA片段化是细胞凋亡的生化标志。这表明蚕血淋巴抑制杆状病毒诱导的昆虫细胞凋亡。然而，蚕血淋巴并不影响假设靶标的数量，这代表宿主细胞对杆状病毒的易感性。培养基中胎牛血清（FBS）的浓度不影响延迟时间，而蚕血淋巴浓度较低导致延迟时间较短。这意味着增加宿主细胞寿命的物质并不存在于胎牛血清中，而是存在于蚕血淋巴中。
The kinetic effect of silkworm hemolymph on host cell viability during a baculovirus-induced insect cell death process was investigated. Host cell viability after viral infection is important for replication of the baculovirus DNA containing a recombinant gene and expression of the cloned gene. The baculovirus-induced insect cell death process can be divided into a delay phase and a first-order death phase, which are characterized by a delay time(t(d)) and a specific death rate (k(d)), respectively. For 0-10% silkworm hemolymph in the media, higher concentrations resulted in longer delay times and lower specific death rates. By adding 10% silkworm hemolymph, the delay time increased from 72 to 164 h, and the specific death rate was reduced from 13.8 x 10(-3) to 6.0 x 10(-3) h(-1). In addition, host cell viability correlated with DNA fragmentation, which is the biochemical hallmark of apoptosis. This indicates that the silkworm hemolymph inhibits the baculovirus-induced insect cell apoptosis. However, the silkworm hemolymph did not affect the number of hypothetical targets, which represents host cell susceptibility to the baculovirus. The concentration of fetal bovine serum (FBS) in the medium did not affect the delay time, while lower concentrations of silkworm hemolymph resulted in shorter delay times. This means that the substance which increases the longevity of the host cell is not in the FBS but in the silkworm hemolymph.