The role of electrical and jasmonate signalling in the recognition of captured prey in the carnivorous sundew plant Drosera capensis
The role of electrical and jasmonate signalling in the recognition of captured prey in the carnivorous sundew plant Drosera capensis
复制标题
DOI：
10.1111/nph.14352
复制
发表时间：
2017-03-01
期刊：
影响因子：
9.4
通讯作者：
Pavlovic, Andrej
中科院分区：
文献类型：
--
作者：
Krausko, Miroslav;Perutka, Zdenek;Pavlovic, Andrej
肉食性太阳露植物(Drosera Capensis)用粘性触须捕捉猎物。我们研究了不同刺激对触须和茉莉酸信号的反应，以揭示肉食性太阳露是如何识别其陷阱中可消化的猎物的。我们测量了局部和系统陷阱中机械刺激、伤害或昆虫取食时的电信号、植物激素浓度、酶活性和Chla荧光。用质谱仪鉴定了消化液中的7种新蛋白质。机械刺激和活体猎物在应用地点诱导了快速、局部的触须弯曲反应和酶分泌。相比之下，反复创伤在局部和远处的全身陷阱中诱导了非局部性的惊厥触角运动和酶的分泌。这些差异可以用电信号传播和茉莉酸积累来解释，这也对陷阱中的光合作用产生了重大影响。伤害产生的电信号可以部分模仿对挣扎中的猎物的机械刺激，并可能触发错误警报，证实植物食肉和植物防御机制是相关的。为了触发完整的酶活性，诱捕器必须从捕获的猎物中检测到化学刺激。
The carnivorous sundew plant (Drosera capensis) captures prey using sticky tentacles. We investigated the tentacle and trap reactions in response to the electrical and jasmonate signalling evoked by different stimuli to reveal how carnivorous sundews recognize digestible captured prey in their traps.We measured the electrical signals, phytohormone concentration, enzyme activities and Chla fluorescence in response to mechanical stimulation, wounding or insect feeding in local and systemic traps. Seven new proteins in the digestive fluid were identified using mass spectrometry.Mechanical stimuli and live prey induced a fast, localized tentacle-bending reaction and enzyme secretion at the place of application. By contrast, repeated wounding induced a nonlocalized convulsive tentacle movement and enzyme secretion in local but also in distant systemic traps. These differences can be explained in terms of the electrical signal propagation and jasmonate accumulation, which also had a significant impact on the photosynthesis in the traps.The electrical signals generated in response to wounding could partially mimic a mechanical stimulation of struggling prey and might trigger a false alarm, confirming that the botanical carnivory and plant defence mechanisms are related. To trigger the full enzyme activity, the traps must detect chemical stimuli from the captured prey.