High performance displacement chromatography of proteins: Separation of β-Lactoglobulins A and B
High performance displacement chromatography of proteins: Separation of β-Lactoglobulins A and B
复制标题
蛋白质的高效置换色谱：β-乳球蛋白 A 和 B 的分离
DOI：
10.1007/bf02688603
复制
发表时间：
1987
期刊：
影响因子：
1.7
通讯作者：
C. Horváth
中科院分区：
文献类型：
--
作者：
A. Liao;Z. Rassi;D. LeMaster;C. Horváth
在阴离子交换柱上，以硫酸软骨素为置换剂，采用高效置换色谱法分离了β-乳球蛋白A和B。在单次色谱运行中，在75×7.5 mm的柱上分离含有两种β-乳葡聚糖混合物的100 mg样品。分离过程遵循经典位移发展规律：两种蛋白质形成连续的矩形带，其浓度依赖于位移浓度。结果表明，高效位移色谱是一种有用的技术，用于分离蛋白质的制备量，柱和仪器通常用于分析HPLC。此外，它有可能成为大规模分离蛋白质的首选方法。
Summaryβ-Lactoglobulins A and B were separated by high performance displacement chromatography on an anion-exchanger column with chondroitin sulfate as the displacer. A sample of 100 mg containing a mixture of the two β-lactoglublins was separated on a column of 75×7.5 mm in a single chromatographic run. The separation process followed the rules of the classical displacement development: the two proteins formed contiguous rectangular bands and their concentrations were dependent on the displacer concentration. The results demonstrate that high performance displacement chromatography is a useful technique for the separation of proteins in preparative amounts with columns and instrumentation typically used in analytical HPLC. Furthermore, it has the potential to become the method of choice in large scale separation of proteins.
串联使用羧肽酶 Y 反应器和置换色谱仪进行肽合成。
DOI：
10.1016/s0021-9673(01)94182-2
发表时间：
1987
期刊：
Journal of chromatography
影响因子：
--
作者：
Cramer,SM;elRassi,Z;Horváth,C
通讯作者：
Horváth,C