Using microcontact printing to pattern the attachment of mammalian cells to self-assembled monolayers of alkanethiolates on transparent films of gold and silver
Using microcontact printing to pattern the attachment of mammalian cells to self-assembled monolayers of alkanethiolates on transparent films of gold and silver
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DOI：
10.1006/excr.1997.3668
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发表时间：
1997-09-15
影响因子：
3.7
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Mrksich, M;Dike, LE;Whitesides, GM
这篇论文描述了一种用于细胞培养的图案化底物的简便方法，该方法允许控制附着细胞的位置和尺寸。该方法使用末端取代烷基硫醇(R(CH2)(11-15)S-)的自组装单分子膜(SAM)吸附在金或银的光学透明膜上来控制底物的性质。端基为甲基的自组装膜吸附蛋白质，端基为低聚乙二醇基的自组装膜完全抵抗蛋白质的吸附。这种方法使用微接触印刷(Mu CP)-一种实验上简单的非光刻工艺-在微米尺度上对SAM的形成进行图案化；Mu CP使用在其表面具有浮雕图案的弹性印章，以相同图案将烷硫醇转移到金或银的表面。制备具有10、30、60和90微米宽的疏水性、甲基端接线并被120微米宽的蛋白质抗性区域隔开的图案化自组装膜，并用纤维连接蛋白包覆；蛋白质仅吸附到甲基终止区域。牛毛细血管内皮细胞仅附着在有图案的自组装膜的纤维连接蛋白涂层的甲基末端区域。这些细胞仍然附着在自体膜上，并被限制在底层自体膜的模式中至少5-7天。由于底物是光学透明的，用荧光素标记的鬼臼毒素固定和染色后，细胞可以在倒置显微镜和荧光显微镜下观察到。(C)1997年学术出版社。
This paper describes a convenient methodology for patterning substrates for cell culture that allows the positions and dimensions of attached cells to be controlled. The method uses self-assembled monolayers (SAMs) of terminally substituted alkanethiolates (R(CH2)(11-15)S-) adsorbed on optically transparent films of gold or silver to control the properties of the substrates. SAMs terminated in methyl groups adsorb protein and SAMs terminated in oligo(ethylene glycol) groups resist entirely the adsorption of protein. This methodology uses microcontact printing (mu CP)-an experimentally simple, nonphotolithographic process-to pattern the formation of SAMs at the micrometer scale; mu CP uses an elastomeric stamp having at its surface a pattern in relief to transfer an alkanethiol to a surface of gold or silver in the same pattern. Patterned SAMs having hydrophobic, methyl-terminated lines 10, 30, 60, and 90 mu m in width and separated by protein-resistant regions 120 mu m in width were prepared and coated with fibronectin; the protein adsorbed only to the methyl-terminated regions. Bovine capillary endothelial cells attached only to the fibronectin-coated, methyl-terminated regions of the patterned SAMs. The cells remained attached to the SAMs and confined to the pattern of underlying SAMs for at least 5-7 days. Because the substrates are optically transparent, cells could be visualized by inverted microscopy and by fluorescence microscopy after fixing and staining with fluorescein-labeled phalloidin. (C) 1997 Academic Press.