Molecular cloning and characterization of chitinase genes from Streptomyces lividans 66
Molecular cloning and characterization of chitinase genes from Streptomyces lividans 66
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浅青紫链霉菌 66 几丁质酶基因的分子克隆和表征
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
Y. Sawada
中科院分区：
文献类型：
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作者：
K. Miyashita;T. Fujii;Y. Sawada
Summary: In order to study the genetic control of chitinolytic activity in Streptomyces, chitinase genes were cloned from S. lividans TK64 into the multicopy plasmid pIJ702 and their expression monitored in their natural host by measuring increases in chitinase productivity. Four independent clones were obtained, and the plasmids named pEMJ1, pEMJ5, pEMJ7 and pEMJ8. Restriction endonuclease digestion showed that although two of the plasmids (pEMJ7 and pEMJ8) shared a common DNA fragment, there were no substantial similarities between the inserts of plasmids pEMJ1, pEMJ5 and pEMJ7. This was confirmed by DNA-DNA hybridization studies. Four chitinases (A, B, C, and D) were identified, with molecular masses of 36, 46, 65, and 41 kDa, respectively. Production of chitinases A and B was specified by the plasmids pEMJ1 and pEMJ5, respectively. Genes for the other two chitinases (C and D) were carried by plasmid pEMJ7. Although significant differences were observed between chitinases A, B, and C in terms of optimum pH for activity and mode of digestion of substrates, chitinases C and D were very similar in these respects. Cloned genes were also expressed in S. coelicolor M130 and in Escherichia coli.
褶皱链霉菌几丁质酶 (chitinase-63) 在大肠杆菌中的克隆和表达。
DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Robbins,PW;Albright,C;Benfield,B
通讯作者：
Benfield,B