Unexpected influence of a C-terminal-fused His-tag on the processing of an enzyme and on the kinetic and folding parameters
Unexpected influence of a C-terminal-fused His-tag on the processing of an enzyme and on the kinetic and folding parameters
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DOI：
10.1016/s0014-5793(97)00908-3
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发表时间：
1997-08-18
期刊：
影响因子：
3.5
通讯作者：
Frere, JM
中科院分区：
文献类型：
--
作者：
Ledent, P;Duez, C;Frere, JM
The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N-terminus, showing that this type of protein engineering might not always be as 'neutral' as generally assumed. (C) 1997 Federation of European Biochemical Societies.
