High removal efficiency of antibiotic resistance genes in swine wastewater via nanofiltration and reverse osmosis processes
High removal efficiency of antibiotic resistance genes in swine wastewater via nanofiltration and reverse osmosis processes
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通过纳滤和反渗透工艺高效去除猪废水中的抗生素抗性基因
DOI：
10.1016/j.jenvman.2018.10.073
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发表时间：
2019-02-01
影响因子：
8.7
通讯作者：
Liu, Dezhao
中科院分区：
文献类型：
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作者：
Lan, Lihua;Kong, Xianwang;Liu, Dezhao
猪场污水处理厂已成为抗生素耐药基因（ARGs）的主要来源之一。膜处理工艺是去除新兴污染物的有前途的解决方案。然而，有限的研究已经调查了纳滤和反渗透处理在去除猪废水中的ARGs的效果。在这项研究中，常见的ARGs，包括sul 1，sul 2，tetA，tetM和tetW，以及intI 1和16 S rRNA基因的存在和命运，在一个中型（6500）猪场污水处理厂（WWTP）配备了常规生物处理和先进的膜处理系统。所有的基因在原污水中都被检测到了高丰度。猪场污水处理厂的生物处理并没有减少ARGs的数量。正如预期的那样，纳滤和反渗透处理有效地减少了ARG的绝对基因拷贝数（与原污水相比，去除了4.98-9.52个对数）。与反渗透出水相比，人工湿地中ARGs的绝对丰度增加了1.00-2.06 log。磺胺类耐药基因相对丰度基本不变，四环素类耐药基因tetA、tedM和tetW的相对丰度分别下降了0.88、3.47和2.51 log。结果表明，先进的膜处理能够有效地去除各种ARGs，以及一些常见的氮和磷污染物。本研究提出了一个成熟的替代方法去除ARGs的牲畜废水。
Swine wastewater treatment plant has become one of the main sources of antibiotic resistance genes (ARGs). Membrane treatment processes are promising solutions for removal of the emerging contaminants. However, limited studies have investigated the effects of nanofiltration and reverse osmosis treatment in removing ARGs in swine wastewater. In this study, the presence and the fate of common ARGs including sul1, sul2, tetA, tetM and tetW, as well as intI1 and 16S rRNA gene, were investigated in a medium-sized (6500) pig farm wastewater treatment plant (WWTP) equipped with conventional biological treatment and advanced membrane processing system. All of the genes were detected with highly abundance in the raw sewage. The biological treatments of the swine wastewater treatment plant did not reduce the quantity of the ARGs. As expected, nanofiltration and reverse osmosis treatment reduced the absolute gene copy number of ARGs efficiently (4.98-9.52 logs removal compared to raw sewage). Compared to the reverse osmosis effluent, however, the absolute abundance of ARGs in the artificial wetland increased by 1.00-2.06 logs. Meanwhile, the relative abundance of sulfonamide resistant genes were basically unchanged, while tetracycline resistance genes (tetA, tedM and tetW) decreased by 0.88, 3.47, 2.51 log, respectively. The results demonstrated that advanced membrane treatments are capable of removing various kinds of ARGs efficiently, as well as some common nitrogen and phosphorus contaminants. This study suggested a mature alternative method for the removal of ARGs from livestock wastewater.