Combinatorial Low-Volume Synthesis of Well-Defined Polymers by Enzyme Degassing
Combinatorial Low-Volume Synthesis of Well-Defined Polymers by Enzyme Degassing
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DOI：
10.1002/anie.201600112
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发表时间：
2016-03-24
影响因子：
16.6
通讯作者：
Stevens, Molly M.
中科院分区：
文献类型：
--
作者：
Chapman, Robert;Gormley, Adam J.;Stevens, Molly M.
长期以来，以低体积、组合的方式合成定义明确的聚合物一直是聚合物化学的目标。在这里，我们报道了在开放的气氛中，通过酶辅助的可逆加成-断裂链转移(Enz-RAFT)聚合在微滴定板中制备了一系列高度可控的均相和嵌段共聚物。在水/溶剂混合物中加入1M葡萄糖氧化酶(GOx)，可以使聚合反应在极低的体积(40L)和极低的自由基浓度下进行。该方法在一系列单体系列和相对分子质量上提供了出色的控制和高转化率，从而避免了在筛选应用中需要进行纯化。这种简单的技术能够在台面上的微滴定板中进行组合聚合物合成，而不需要高度专业化的合成器，而且合成数量比目前任何其他技术都要低得多。
The synthesis of well-defined polymers in a low-volume, combinatorial fashion has long been a goal in polymer chemistry. Here, we report the preparation of a wide range of highly controlled homo and block co-polymers by Enz-RAFT (enzyme-assisted reversible addition-fragmentation chain transfer) polymerization in microtiter plates in the open atmosphere. The addition of 1m glucose oxidase (GOx) to water/solvent mixtures enables polymerization reactions to proceed in extremely low volumes (40L) and low radical concentrations. This procedure provides excellent control and high conversions across a range of monomer families and molecular weights, thus avoiding the need to purify for screening applications. This simple technique enables combinatorial polymer synthesis in microtiter plates on the benchtop without the need of highly specialized synthesizers and at much lower volumes than is currently possible by any other technique.