A Val-202-Phe α-tubulin mutation and enhanced metabolism confer dinitroaniline resistance in a single Lolium rigidum population
A Val-202-Phe α-tubulin mutation and enhanced metabolism confer dinitroaniline resistance in a single Lolium rigidum population
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DOI：
10.1002/ps.5561
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发表时间：
2019-08-15
影响因子：
4.1
通讯作者：
Powles, Stephen B.
中科院分区：
文献类型：
--
作者：
Chen, Jinyi;Chu, Zhizhan;Powles, Stephen B.
先前报道了从西澳大利亚收集的硬直黑麦草种群对二硝基苯胺除草剂具有高度抗性，主要是由于靶位点α-微管蛋白中的瓦尔-202-Phe取代。为了进一步确定202突变对抗性的贡献，从同一抗性群体中分离分别包含202突变体和野生型（WT）个体的两个亚群体，并使其经受二硝基苯胺除草剂剂量。进行水稻转基因研究以证明在202残基处的氨基酸取代是否赋予抗性。此外，如表型和基因型研究所示，在同一人群中进一步检查了非靶向增强的氟乐灵代谢。结果202株突变体的抗性均高于野生型。将L.相对于用野生型基因转化的愈伤组织，刚性突变体α-微管蛋白基因（瓦尔-202-Phe）对二硝基苯胺除草剂具有更高的抗性。此外，增强氟乐灵代谢检测到202突变体相比，敏感的幼苗。结论在二硝基苯胺耐药菌株中同时存在靶位点瓦尔-202-Phe α-微管蛋白突变和非靶位点氟乐灵代谢增强。刚性种群(c)2019化学工业协会
BACKGROUND A Lolium rigidum population collected from Western Australia was previously reported as highly resistant to dinitroaniline herbicides mainly due to a Val-202-Phe substitution in the target site alpha-tubulin protein. To further determine the contribution of the 202 mutation to resistance, two sub-populations, respectively comprising the 202 mutant and wild-type (WT) individuals, were isolated from within the same resistant population and subject to dinitroaniline herbicide doses. A rice transgenic study was conducted to demonstrate whether the amino acid substitution at the 202 residue confers resistance. In addition, as indicated in the phenotyping and genotyping study, non-target enhanced trifluralin metabolism was further examined in the same population. RESULTS The 202 mutants were more resistant than the wild-type plants. Rice calli transformed with the L. rigidum mutant alpha-tubulin gene (Val-202-Phe) were more resistant to dinitroaniline herbicides relative to calli transformed with the wild-type gene. Also, enhanced trifluralin metabolism was detected in the 202 mutants in comparison to the susceptible seedlings. CONLCUSION Both target-site Val-202-Phe alpha-tubulin mutation and non-target-site enhanced trifluralin metabolism co-exist in this dinitroaniline-resistant L. rigidum population. (c) 2019 Society of Chemical Industry