Monoclonal antibodies that detect different antigenic determinants of the same human osteosarcoma-associated antigen.
Monoclonal antibodies that detect different antigenic determinants of the same human osteosarcoma-associated antigen.
复制标题
检测同一人骨肉瘤相关抗原的不同抗原决定簇的单克隆抗体。
作者：
T. Wada;T. Uede;S. Ishii;K. Matsuyama;S. Yamawaki;K. Kikuchi
用人骨肉瘤细胞系免疫BALB/c小鼠，取其脾细胞与NS/1小鼠骨髓瘤细胞融合，获得抗人骨肉瘤细胞的单克隆抗体。建立了两个杂种无性系，命名为2 H10和2D 3。这两种单克隆抗体与所有骨肉瘤组织反应强烈，但不与其他骨和软组织肿瘤，如软骨肉瘤，恶性纤维组织细胞瘤，脂肪肉瘤，平滑肌肉瘤，横纹肌肉瘤。此外，两种单克隆抗体均不与肿瘤细胞系和从其他癌症中获得的组织反应。免疫组化显示2 H10和2D 3与肉瘤组织中的内皮细胞反应，而与其他肿瘤和正常组织中的内皮细胞不反应。2 H10还与皮肤表皮基底层上的细胞反应。2 H10和2D 3定义的抗原在非还原和还原条件下的分子量约为75，000，表明抗原具有单链结构，不存在分子内二硫键。2 H10和2D 3定义的抗原的pI值在5.5和6.2之间。连续免疫沉淀分析清楚地表明2 H10和2D 3识别相同的抗原分子。然而，进一步的分析表明，2 H10和2D 3单克隆抗体识别相同抗原分子上的不同抗原决定簇的可能性。
Monoclonal antibodies (MoAbs) against human osteosarcoma cells were obtained by the fusion of NS/1 mouse myeloma cells with spleen cells from the human osteosarcoma cell line-immunized BALB/c mice. Two hybrid clones were established and designated as 2H10 and 2D3. Both MoAbs reacted strongly with all osteosarcoma tissues but not with other bone and soft tissue tumors such as chondrosarcoma, malignant fibrous histiocytoma, liposarcoma, leiomyosarcoma, and rhabdomyosarcoma. In addition, neither MoAb reacted with tumor cell lines and tissues obtained from other cancers. Immunohistochemical analysis demonstrated that 2H10 and 2D3 reacted with endothelial cells in sarcoma tissues, but not with those of other tumors and normal tissues. 2H10 also reacted with cells on the basal layer of epidermis of the skin. 2H10- and 2D3-defined antigen has an approximate molecular weight of 75,000 under nonreducing and reducing conditions, indicating that the antigen has a single chain structure and there is no intramolecular disulfide bond. 2H10- and 2D3-defined antigen has a pI value between 5.5 and 6.2. Sequential immunoprecipitation analysis clearly demonstrated that 2H10 and 2D3 recognized the same antigen molecule. However, further analysis suggested the possibility that 2H10 and 2D3 MoAbs recognized the different antigenic determinants on the same antigen molecule.