Forkhead box P3 (FOXP3) mRNA expression immediately after living-donor liver transplant.
Forkhead box P3 (FOXP3) mRNA expression immediately after living-donor liver transplant.
复制标题
活体肝移植后叉头盒 P3 (FOXP3) mRNA 立即表达。
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Y. Inomata
中科院分区：
文献类型：
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作者：
Rieko Sakamoto;K. Asonuma;Manuel E. Zeledon Ramirez;K. Yoshimoto;Aya Nishimori;Y. Inomata
目标
FOXP3基因被认为是调节性T细胞的主基因。调节性T细胞在肝移植中的意义在以前的报道中已经研究过，但是活体肝移植后FOXP3信使RNA（mRNA）的定量表达尚未评估。本研究的目的是确定人类FOXP3基因是否是活体肝移植受者在移植后即刻T细胞调节活性的良好标志物。
材料和方法
在移植后的第一个月内，在15例活体肝移植受者的外周血单核细胞中，我们使用流式细胞分选术测量了CD4+CD25+ T细胞的数量，并使用实时聚合酶链反应测量了FOXP3 mRNA的表达。
结果
与术前参考值相比，术后第7天FOXP3 mRNA的诱导倍数显著增加（3.3倍）（P <0.01），但在移植后28天恢复至基线。CD4+CD25+ T细胞的数量没有显著变化。在活体肝移植后60天内发生急性细胞排斥反应的受者中，FOXP3 mRNA在第14、21和28天的表达较低。
结论
活体肝移植后即刻FOXP3 mRNA表达的增加可能受到包括调节性T细胞和其他T细胞在内的T细胞活化的影响。然而，在这些激活曲线稳定后，FOXP3mRNA的表达似乎与移植物的接受有关。进一步的研究是必要的，在适当的采样点测量FOXP3 mRNA的表达。
OBJECTIVES
The forkhead box P3 (FOXP3) gene is considered to be the master gene of regulatory T cells. The significance of regulatory T cells in liver transplant has been investigated in previous reports, but quantitative FOXP3 messenger RNA (mRNA) expression after living-donor liver transplant has not been assessed. The objective of this study was to determine whether the human FOXP3 gene is a good marker for regulatory activity in T cells in living-donor liver transplant recipients during the immediate posttransplant period.
MATERIALS AND METHODS
In peripheral blood mononuclear cells of 15 living-donor liver transplant recipients during the first month after transplant; we measured the population of CD4+CD25+ T cells using flow-assisted cell sorting and the expression of FOXP3 mRNA using real-time polymerase chain reaction.
RESULTS
Fold induction of FOXP3 mRNA significantly increased on postoperative day 7 (3.3-fold) compared with the reference preoperative value (P < .01) but returned to baseline by 28 days after transplant. The population of CD4+CD25+ T cells did not change significantly. Expression of FOXP3 mRNA on days 14, 21, and 28 were lower in recipients with acute cellular rejection within 60 days after living-donor liver transplant.
CONCLUSIONS
Increased expression of FOXP3 mRNA immediately after living-donor liver transplant might be influenced by activation of T cells including regulatory T cells and other T cells. However, after stabilization of these activation profiles, it seems likely that FOXP3mRNA expression is associated with graft acceptance. Further studies are necessary with measurement of FOXP3 mRNA expression at appropriate sampling points.