Single-cell analysis of immune cell transcriptome during HIV-1 infection and therapy.
Single-cell analysis of immune cell transcriptome during HIV-1 infection and therapy.
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DOI：
10.1186/s12865-022-00523-2
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发表时间：
2022-09-29
期刊：
影响因子：
3
通讯作者：
中科院分区：
文献类型：
--
作者：
Cellular immune responses are phenotypically and functionally perturbed during HIV-1 infection, with the majority of function restored upon antiretroviral therapy (ART). Despite ART, residual inflammation remains that can lead to HIV-related co-morbidities and mortality, indicating that ART does not fully restore normal immune cell function. Thus, understanding the dynamics of the immune cell landscape during HIV-1 infection and ART is critical to defining cellular dysfunction that occurs during HIV-1 infection and imprints during therapy. Here, we have applied single-cell transcriptome sequencing of peripheral blood immune cells from chronic untreated HIV-1 individuals, HIV-1-infected individuals receiving ART and HIV-1 negative individuals. We also applied single-cell transcriptome sequencing to a primary cell model of early HIV-1 infection using CD4+ T cells from healthy donors. We described changes in the transcriptome at high resolution that occurred during HIV-1 infection, and perturbations that remained during ART. We also determined transcriptional differences among T cells expressing HIV-1 transcripts that identified key regulators of HIV-1 infection that may serve as targets for future therapies to block HIV-1 infection. This work identified key molecular pathways that are altered in immune cells during chronic HIV-1 infection that could remain despite therapy. We also identified key genes that are upregulated during early HIV-1 infection that provide insights on the mechanism of HIV-1 infection and could be targets for future therapy. The online version contains supplementary material available at 10.1186/s12865-022-00523-2.
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影响因子：
3.8
作者：
Kotlarz, Agnieszka;Tukaj, Stefan;Krzewski, Konrad;Brycka, Elzbieta;Lipinska, Barbara
通讯作者：
Lipinska, Barbara
DOI：
10.1056/nejmoa1600693
发表时间：
2016-09-01
期刊：
The New England journal of medicine
影响因子：
--
作者：
Cohen MS;Chen YQ;McCauley M;Gamble T;Hosseinipour MC;Kumarasamy N;Hakim JG;Kumwenda J;Grinsztejn B;Pilotto JH;Godbole SV;Chariyalertsak S;Santos BR;Mayer KH;Hoffman IF;Eshleman SH;Piwowar-Manning E;Cottle L;Zhang XC;Makhema J;Mills LA;Panchia R;Faesen S;Eron J;Gallant J;Havlir D;Swindells S;Elharrar V;Burns D;Taha TE;Nielsen-Saines K;Celentano DD;Essex M;Hudelson SE;Redd AD;Fleming TR;HPTN 052 Study Team
通讯作者：
HPTN 052 Study Team
影响因子：
8.8
作者：
Bradley, Todd;Ferrari, Guido;Browne, Edward P.
通讯作者：
Browne, Edward P.
DOI：
10.1073/pnas.2035075100
发表时间：
2003-12-09
影响因子：
11.1
作者：
Abdelwahab, SF;Cocchi, F;Lewis, GK
通讯作者：
Lewis, GK
影响因子：
7.7
作者：
Brodin, Johanna;Zanini, Fabio;Albert, Jan
通讯作者：
Albert, Jan