Establishment of histone H3 methylation on the inactive X chromosome requires transient recruitment of Eed-Enx1 Polycomb group complexes
Establishment of histone H3 methylation on the inactive X chromosome requires transient recruitment of Eed-Enx1 Polycomb group complexes
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DOI：
10.1016/s1534-5807(03)00068-6
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发表时间：
2003-04-01
影响因子：
11.8
通讯作者：
Brockdorff, N
中科院分区：
文献类型：
--
作者：
Silva, J;Mak, W;Brockdorff, N
先前的研究表明Eed-Enx 1 Polycomb组复合物在小鼠滋养外胚层谱系中维持印迹X失活。在这里，我们表明，招聘Eed-Enx 1的失活X染色体（Xi）也发生在随机X失活在胚胎适当的。Eed-Enx 1复合物在Xi上的定位发生得非常早，在Xist表达开始时，但随后随着分化和发育的进展而消失。这种瞬时定位与全能细胞中和早期分化阶段高水平复合物的存在相关。功能分析表明，Eed-Enx 1是需要建立组蛋白H3的甲基化在赖氨酸9和/或赖氨酸27上的Xi，这反过来又是需要稳定的Xi染色质结构。
Previous studies have implicated the Eed-Enx1 Polycomb group complex in the maintenance of imprinted X inactivation in the trophectoderm lineage in mouse. Here we show that recruitment of Eed-Enx1 to the inactive X chromosome (Xi) also occurs in random X inactivation in the embryo proper. Localization of Eed-Enx1 complexes to Xi occurs very early, at the onset of Xist expression, but then disappears as differentiation and development progress. This transient localization correlates with the presence of high levels of the complex in totipotent cells and during early differentiation stages. Functional analysis demonstrates that Eed-Enx1 is required to establish methylation of histone H3 at lysine 9 and/or lysine 27 on Xi and that this, in turn, is required to stabilize the Xi chromatin structure.