Genome Wide Association Mapping for the Tolerance to the Polyamine Oxidase Inhibitor Guazatine in Arabidopsis thaliana.
Genome Wide Association Mapping for the Tolerance to the Polyamine Oxidase Inhibitor Guazatine in Arabidopsis thaliana.
复制标题
基因组宽的缔合图形，以拟南芥中对多胺氧化酶抑制剂番茄的耐受性。
DOI：
10.3389/fpls.2016.00401
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发表时间：
2016
影响因子：
5.6
通讯作者：
Alcázar R
中科院分区：
文献类型：
--
作者：
Atanasov KE;Barboza-Barquero L;Tiburcio AF;Alcázar R
胍丁胺是一种有效的多胺氧化酶（PAO）活性抑制剂。在农业中，胍菌素被用作非内吸性接触杀真菌剂，有效保护谷物和柑橘类水果免受疾病的侵害。胍扎汀的组成很复杂，主要由合成的胍基多胺（聚氨基胍）的混合物组成。在这里，我们已经研究了暴露于胍在杂草拟南芥的影响。我们报告说，微摩尔浓度的胍盐足以抑制拟南芥幼苗的生长和诱导褪绿，而发芽几乎没有影响。我们观察到发生的数量变化之间的反应，以胍盐107随机选择的拟南芥加入。这使我们能够进行全基因组关联（GWA）定位，确定一号染色体上与胍丁胺耐受性相关的基因座。该基因座内的叶绿素酶1（CLH 1）及其副产物（CLH 2）作为候选基因进行了研究。对独立的clh 1 -2、clh 1 -3、clh 2 -3、clh 2 -2和双clh 1 -2 clh 2 -3突变等位基因的分析表明，CLH 1和/或CLH 2功能丧失或表达下调促进了拟南芥中胍盐耐受性。我们报告了一个自然的机制，拟南芥种群可以克服毒性的杀真菌剂胍。
Guazatine is a potent inhibitor of polyamine oxidase (PAO) activity. In agriculture, guazatine is used as non-systemic contact fungicide efficient in the protection of cereals and citrus fruits against disease. The composition of guazatine is complex, mainly constituted by a mixture of synthetic guanidated polyamines (polyaminoguanidines). Here, we have studied the effects from exposure to guazatine in the weed Arabidopsis thaliana. We report that micromolar concentrations of guazatine are sufficient to inhibit growth of Arabidopsis seedlings and induce chlorosis, whereas germination is barely affected. We observed the occurrence of quantitative variation in the response to guazatine between 107 randomly chosen Arabidopsis accessions. This enabled us to undertake genome-wide association (GWA) mapping that identified a locus on chromosome one associated with guazatine tolerance. CHLOROPHYLLASE 1 (CLH1) within this locus was studied as candidate gene, together with its paralog (CLH2). The analysis of independent clh1-2, clh1-3, clh2-3, clh2-2, and double clh1-2 clh2-3 mutant alleles indicated that CLH1 and/or CLH2 loss-of-function or expression down-regulation promote guazatine tolerance in Arabidopsis. We report a natural mechanism by which Arabidopsis populations can overcome toxicity by the fungicide guazatine.