Changes in aberrant DNA methylation after Helicobacter pylori eradication: A long-term follow-up study
Changes in aberrant DNA methylation after Helicobacter pylori eradication: A long-term follow-up study
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DOI：
10.1002/ijc.28219
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发表时间：
2013-11-01
影响因子：
6.4
通讯作者：
Jung, Hyun Chae
中科院分区：
文献类型：
--
作者：
Shin, Cheol Min;Kim, Nayoung;Jung, Hyun Chae
根除幽门螺杆菌后胃粘膜DNA甲基化的变化尚未阐明。在此背景下，我们研究了 221 名成功根除幽门螺杆菌的受试者，并通过内镜随访至少 6 个月，研究了根除幽门螺杆菌后 DNA 甲基化的时间进程，其中包括 114 名对照者、53 名胃异型增生受试者和 54 名早期胃癌患者。所有不典型增生和胃癌患者在入组时均接受了内镜切除术。使用定量甲基化特异性 PCR 比较非癌性胃粘膜的 LOX、APC 和 MOS 基因的甲基化水平，以及根除前后胃粘膜的组织学结果。平均随访时间为 26.0 个月（范围：6 至 76 个月）。幽门螺杆菌根除降低了 LOX 的甲基化水平（斜率的 p 值）
Changes of DNA methylation in gastric mucosae after eradication of Helicobacter pylori have not been clarified yet. From this background, we investigated time course of DNA methylation following H. pylori eradication in 221 successfully H. pylori eradicated subjects with endoscopic follow-up at least for 6 months, including 114 controls, 53 subjects with gastric dysplasia and 54 patients with early gastric cancer. All dysplasia and gastric cancer patients underwent endoscopic resection at the time of enrollment. The methylation levels in LOX, APC and MOS genes from noncancerous gastric mucosae using quantitative methylation-specific PCR, as well as the histologic findings of gastric mucosae, were compared before and after eradication. Average follow-up duration was 26.0 months (range: 6 to 76 months). H. pylori eradication decreased methylation levels in LOX (p-value for slope