Biocomposite scaffolds for bone regeneration: Role of chitosan and hydroxyapatite within poly-3-hydroxybutyrate-co-3-hydroxyvalerate on mechanical properties and in vitro evaluation.
Biocomposite scaffolds for bone regeneration: Role of chitosan and hydroxyapatite within poly-3-hydroxybutyrate-co-3-hydroxyvalerate on mechanical properties and in vitro evaluation.
复制标题
DOI：
10.1016/j.jmbbm.2015.06.032
复制
发表时间：
2015-11
影响因子：
3.9
通讯作者：
Sai Zhang;M. Prabhakaran;X. Qin;S. Ramakrishna
中科院分区：
文献类型：
--
作者：
Sai Zhang;M. Prabhakaran;X. Qin;S. Ramakrishna
骨组织再生的生物工程支架是一个爆炸性的研究领域，主要是因为它们可以通过模仿天然骨的细胞外基质（ECM）来满足骨替代治疗的基本需求和当前挑战。在本研究中，我们制备了生物复合纳米纤维支架与聚-3-羟基丁酸酯-co-3-羟基戊酸酯（PHBV），壳聚糖（CTS）和羟基磷灰石（HA）的共混物。PHBV、PHBV/CTS（90：10）、PHBV/CTS/HA 4（85.5：9.5：5）和PHBV/CTS/HA 8（81：9：10）的纤维直径分别为405±74 nm、334±82 nm、316±103 nm和256±110 nm。PHBV/CTS/HA 4和PHBV/CTS/HA 8支架能够承受人胎儿成骨细胞（hFOB）的长期培养，其极限拉伸强度分别为3.55± 0.22MPa和4.19± 0.19MPa。第20天，成骨细胞在PHBV/CTS/HA 8支架上的增殖比在PHBV支架上高34.10%。在第20天通过碱性磷酸酶活性鉴定的细胞成熟在PHBV/CTS/HA 8支架上显著高于在PHBV支架上。EDX分析证实，到第20天，PHBV/CTS/HA 8支架上的细胞也获得了比PHBV支架上的矿物质沉积更高的矿物质沉积（25.79%）。基于这些结果，我们得出结论，静电纺丝PHBV/CTS/HA 8支架具有很大的潜力，以促进骨组织的再生，由于壳聚糖和HA的协同作用，从而壳聚糖提供细胞识别位点，而HA作为螯合剂组织磷灰石样矿化。
Bio-engineered scaffolds for bone tissue regeneration is an exploding area of research mainly because they can satisfy the essential demands and current challenges in bone replacement therapies, by imitating the extracellular matrix (ECM) of the native bone. We fabricated bio-composite nanofibrous scaffolds with a blend of poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV), chitosan (CTS) and hydroxyapatite (HA) during this study. Morphological evaluation confirmed the fiber diameters of PHBV, PHBV/CTS (90:10), PHBV/CTS/HA4 (85.5:9.5:5) and PHBV/CTS/HA8 (81:9:10) as 405±74 nm, 334±82 nm, 316±103 nm and 256±110 nm, respectively. The PHBV/CTS/HA4 and PHBV/CTS/HA8 scaffolds were capable of enduring the long term culture of human fetal osteoblasts (hFOB) with ultimate tensile strength of 3.55±0.22 MPa and 4.19±0.19 MPa, respectively. The proliferation of osteoblasts on PHBV/CTS/HA8 scaffold was found 34.10% higher than that on PHBV scaffold on day 20. Cell maturation identified by alkaline phosphatase activity on day 20 was significantly higher on PHBV/CTS/HA8 scaffold than that on PHBV scaffold. The cells on PHBV/CTS/HA8 scaffold also acquired higher mineral deposition (25.79%) than the mineral deposition on PHBV scaffold by day 20, confirmed by EDX analysis. Based on the results, we concluded that the electrospun PHBV/CTS/HA8 scaffolds hold great potential to promote the regeneration of bone tissue due to the synergistic effect of chitosan and HA, whereby chitosan provided cell recognition sites while HA acted as a chelating agent for organizing the apatite-like mineralization.