Change in Tetrodotoxin Content of the Puffer Fish Takifugu rubripes during Seed Production from Fertilized Eggs to Juveniles
Change in Tetrodotoxin Content of the Puffer Fish Takifugu rubripes during Seed Production from Fertilized Eggs to Juveniles
复制标题
DOI：
10.3358/shokueishi.51.48
复制
发表时间：
2010-02-01
影响因子：
0.3
通讯作者：
Shiomi, Kazuo
中科院分区：
文献类型：
--
作者：
Nagashima, Yuji;Mataki, Isao;Shiomi, Kazuo
研究了河豚鱼种生产过程中河豚毒素(TTX)含量的变化。使用两种含有TTX的成熟雌性河豚鱼(样品1和样品2)。对毒卵进行人工受精，孵化后的幼虫在室内水箱中饲养50天，然后在海上的网箱中再饲养48或38天。样品1受精卵的TTX含量最初为13.0微克TTX/g，在孵化后4天瞬间增加到67.6微克TTX/g，然后逐渐下降到98天时的0.28微克TTX/g。受精期为0.016-0.03mgTTX，幼虫期为0.016-0.03mgTTX，直至孵化后30d。此后，在海上网箱养殖过程中，总TTX含量显著增加，98d时TTX总量达到4.80 mgTTX。样品2的TTX含量变化趋势与样品1相似。本研究表明，从受精卵到幼鱼的过程中，每克河豚体重的TTX含量下降，而总TTX含量增加。
Changes in tetrodotoxin (TTX) content of the puffer fish Takifugu rubripes during seed production were examined. Two mature female puffer fish T. rubripes (samples 1 and 2) that contained TTX were used. The toxic eggs were artificially fertilized, and hatchlings were reared in an indoor tank for 50 days and then in a netcage at sea for an additional 48 or 38 days. The TTX content of the fertilized eggs of sample 1 was initially 13.0 mu g TTX/g, transiently increased to 67.6 mu g TTX/g at 4 days after hatching, and then gradually decreased to 0.28 mu g TTX/g at 98 days. In contrast, the total TTX content in an individual was 0.016 mu g TTX at the fertilization stage and 0.01-0.03 mu g TTX at the larval stage until 30 days after hatching. Thereafter, the total TTX content increased remarkably during culture in the netcage at sea, reaching 4.80 mu g TTX at 98 days. Change in the TTX content of sample 2 showed a similar tendency to that of sample 1. The present study showed that the TTX content per gram of puffer fish body weight decreased during progression from fertilized eggs to juveniles, whereas the total TTX content increased.