Caenorhabditis elegans contains a third Polo-like kinase gene
Caenorhabditis elegans contains a third Polo-like kinase gene
复制标题
DOI：
10.3109/10425170009033251
复制
发表时间：
2000-01-01
期刊：
影响因子：
--
通讯作者：
Ferris, DK
中科院分区：
文献类型：
--
作者：
Chase, D;Golden, A;Ferris, DK
Polo家族的丝氨酸/苏氨酸激酶在许多不同的生物体中参与细胞周期控制。它们的定位和生化活性表明，它们在中心体成熟、G2-M期进展、后期促进和胞质分裂过程中发挥着重要作用。Polo家族与其他丝氨酸/苏氨酸激酶的不同之处在于，它们的非催化C-末端结构域都含有Polo-box序列基序。最近，有报道称线虫体内存在两种Polo相关的蛋白激酶，即plc1和plc2。Plc2与plc1分离，Polo-box序列同源性较差，与plc1的氨基酸同源性只有40%。我们在此报道了线虫另一种Polo相关蛋白的全长cdna序列。预测的蛋白产物与PLK-1/Plc1的氨基酸同源性大于70%，并且具有高度保守的Polo-box结构域。
The Polo family of serine/ threonine kinases have been implicated in cell cycle control in a number of diverse organisms. Their localization and biochemical activity suggest that they play an important role in centrosome maturation, G2-to-M phase progression, the promotion of anaphase, and cytokinesis. The Polo family of kinases is distinct from other serine/threonine kinases in that they all contain a polo-box sequence motif in their non-catalytic C-terminal domain. Recently, it was reported that two Polo-related kinases, Plc1 and Plc2, are present in C. elegans. Plc2 has diverged from Plc1 with poor homology within the polo-box sequence and only had 40% amino acid identity with Plc1. We report here the full-length cDNA sequence of another Polo-related kinase from C. elegans. The predicted protein product has greater than 70% amino acid identity with PLK-1/Plc1, and has a highly conserved polo-box domain.