Identification of two major loci and linked marker for oil content in peanut (Arachis hypogaea L.)
Identification of two major loci and linked marker for oil content in peanut (Arachis hypogaea L.)
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花生（ArachishypogaeaL.）两个主要位点和含油量连锁标记的鉴定
DOI：
10.1007/s10681-021-02765-4
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发表时间：
2021-01-28
期刊：
影响因子：
1.9
通讯作者：
Jiang, Huifang
中科院分区：
文献类型：
--
作者：
Guo, Jianbin;Liu, Nian;Jiang, Huifang
花生是植物油的主要来源之一。提高含油量是花生育种计划的重要目标。利用与含油量性状连锁的主基因座，可以通过分子标记辅助育种快速培育高油品种。为了确定影响油分含量的主效位点，利用高密度遗传图谱，以中花10号和ICG 12625为材料，对RIL群体进行了QTL分析。在3个环境中共检测到18个加性QTL，其中包括2个主效和稳定QTL（qOCB 06和qOCB10.1）。此外，还将26对具有上位性互作的QTL定位在17个连锁群上。qOCB 06位于B 06的0.6 Mb区间内，表型变异率高达22.59%。B10的qOCB 10.1可解释9.18-12.55%的表型变异。在不同的环境条件下，两个QTL的加性效应范围为0.70 ~ 1.13%。这两个位点的有利等位基因分别来自父本和母本。在RIL群体中聚合这两个有利等位基因可以在多个环境中增加“2.66 ± 0.48%”的含油量。分别与qOCB 06和qOCB10.1连锁的标记Ai 06 B29452和AGGS 2133 -1被验证与具有不同表型的花生种质的含油量相关。这些标记在花生含油量改良的分子标记辅助选择和育种中具有重要的应用价值。
Peanut is one of major sources for vegetable oil. Improving oil content is an important goal for peanut breeding programs. Major loci with linked markers for oil content could facilitate rapid development of high-oil varieties through marker-assisted breeding. To identify major loci underlying the oil content, we conducted the QTL analysis based on the high-dense genetic map, using RIL population derived from Zhonghua10 and ICG12625. A total of 18 additive QTLs, including two major and stable QTLs (qOCB06 and qOCB10.1), were identified in three environments. Besides, 26 pairs of QTLs with epistatic (additive × additive) interaction were mapped on 17 linkage groups. qOCB06 with up to 22.59% of the phenotypic variations was located in a 0.6-Mb interval of B06. qOCB10.1 on B10 explained 9.18–12.55% of the phenotypic variations. The additive effect of two QTLs ranged from 0.70 to 1.13% oil content in different environments. Favorable alleles of the two loci were from male and female parent, respectively. Pyramiding the two favorable alleles in the RIL population could increase “2.66 ± 0.48%” oil content across multiple environments. The marker Ai06B29452 and AGGS2133-1 linked to qOCB06 and qOCB10.1, respectively, were validated to be associated with oil content in peanut germplasms with diverse phenotypes. These markers were valuable in marker-assisted selection and pyramiding breeding for improving oil content in peanut.