The reovirus M1 gene determines the relative capacity of growth of reovirus in cultured bovine aortic endothelial cells.
The reovirus M1 gene determines the relative capacity of growth of reovirus in cultured bovine aortic endothelial cells.
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呼肠孤病毒M1基因决定了呼肠孤病毒在培养的牛主动脉内皮细胞中生长的相对能力。
DOI：
10.1172/jci116910
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Smith,TW
中科院分区：
文献类型：
--
作者：
Matoba,Y;Colucci,WS;Fields,BN;Smith,TW
由于血液传播的病毒通常在组织侵入之前与内皮细胞相互作用，因此病毒与内皮细胞之间的相互作用在病毒致病性中可能是重要的。两种呼肠孤病毒分离株（1型Lang和3型Dearing）在培养的牛主动脉内皮细胞中生长的能力不同。哺乳动物呼肠孤病毒在其基因组中具有10个双链RNA基因片段。通过使用24个重组病毒，观察到不同菌株在培养的内皮细胞中生长的能力差异被映射到M1基因（P = 0.00019），该基因编码病毒核心蛋白μ 2。在两种呼肠孤病毒分离株之间，未检测到亲本病毒粒子的病毒外衣壳蛋白的结合或蛋白水解加工的差异。北方印迹分析表明，3型迪林呼肠孤病毒感染的内皮细胞中的病毒mRNA的生产减少，但不是1型郎。因此，我们已经确定了一个病毒基因（M1基因）负责确定在培养的内皮细胞中的两个呼肠孤病毒分离株的生长能力的差异。呼肠孤病毒是一种有吸引力的模型，可以在分子遗传水平上研究病毒与内皮细胞的相互作用。图片
Since blood-borne viruses often interact with endothelial cells before tissue invasion, the interaction between viruses and endothelial cells is likely to be important in viral pathogenicity. Two reovirus isolates (type 1 Lang and type 3 Dearing) differ in their capacity to grow in cultured bovine aortic endothelial cells. The mammalian reoviruses have 10 double-stranded RNA gene segments in their genome. By using 24 reassortant viruses, observed differences in the capacity of different strains to grow in cultured endothelial cells were mapped to the M1 gene (P = 0.00019), which encodes the viral core protein mu 2. No differences were detected in binding or proteolytic processing of viral outer capsid proteins of parental virions between the two reovirus isolates. Northern blot analysis showed a decreased production of viral mRNA in endothelial cells infected with type 3 Dearing reovirus, but not type 1 Lang. Thus, we have identified a viral gene (the M1 gene) responsible for determining the difference in growth capacity of the two reovirus isolates in cultured endothelial cells. Reovirus is an attractive model in which to study the interaction of viruses with endothelial cells at a molecular genetic level.Images