Possible compensatory role among chloroplast proteases under excess-light stress condition.
Possible compensatory role among chloroplast proteases under excess-light stress condition.
复制标题
DOI：
10.4161/psb.23198
复制
发表时间：
2013-03
影响因子：
2.9
通讯作者：
Sakamoto W
中科院分区：
文献类型：
--
作者：
Kato Y;Sakamoto W
光系统II(PSII)的反应中心蛋白D1被认为是光损伤的主要靶点，通过PSII修复周期有效地修复，以应对持续的光氧化损伤。最近对拟南芥的研究表明，在体内的PSII修复中，内源性的Deg酶和外源性的FtsH酶协同降解D1。特别有趣的是，当FtsH在缺乏FtsH2的突变体中受到限制时，我们观察到CLP和SPPA蛋白酶的上调。为了研究叶绿体蛋白水解酶的互补功能是如何被普遍调控的，我们在野生型拟南芥叶片上进行了高光胁迫。野生型叶片在过强的光照下也表现出这些蛋白水解酶的水平升高，这不仅揭示了FtsH和Deg在PSII修复中的重要性，而且也暗示了在慢性胁迫条件下叶绿体蛋白水解酶之间的合作。
The reaction center protein D1 of photosystem II (PSII), known as a primary target of photodamage, is repaired efficiently by the PSII repair cycle, to cope with constant photooxidative damage. Recent studies of Arabidopsis show that the endo-type Deg protease and the exo-type FtsH proteases cooperatively degrade D1 in the PSII repair in vivo. It is particularly interesting that we observed upregulation of Clp and SppA proteases when FtsH was limited in the mutant lacking FtsH2. To examine how the complementary functions of chloroplastic proteases are commonly regulated, we undertook a high-light stress on wild-type Arabidopsis leaves. The result that wild type leaves also showed increased levels of these proteases upon exposure to excessively strong illumination not only revealed the importance of FtsH and Deg in the PSII repair, but also implied cooperation among chloroplastic proteases under chronic stress conditions.