Nanoparticle-based polyacrylonitrile monolithic column for highly efficient micro solid-phase extraction of carotenoids and vitamins in human serum
Nanoparticle-based polyacrylonitrile monolithic column for highly efficient micro solid-phase extraction of carotenoids and vitamins in human serum
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基于纳米颗粒的聚丙烯腈整体柱，用于高效微固相萃取人血清中的类胡萝卜素和维生素
DOI：
10.1016/j.chroma.2020.461755
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发表时间：
2021-01-04
影响因子：
4.1
通讯作者：
Yu, Yan
中科院分区：
文献类型：
--
作者：
Qi, Fei-Fei;Ma, Tian-You;Yu, Yan
建立了一种基于生物相容整体柱的微固相萃取(MU-SPE)生物体液分析方法。通过热诱导相分离将三十烷基改性的二氧化硅纳米颗粒(NPs)引入聚丙烯腈整体柱中，制备了一种新型的纳米聚丙烯腈整体柱(C30NP-PMC)。C30 NP-PMC具有高效的传质和吸附能力，对人血清样品中类胡萝卜素和脂溶维生素(FSV)的提取表现出优异的性能，优于市售的C18色谱柱和液液萃取(LLE)方法。在最佳条件下，该方法与高效液相色谱-二极管阵列检测器联用，在较低的吸附剂(35 Mg)和有机溶剂(0.8 m L)的用量下，获得了满意的检测限(1.5~75.0 ng/m L)和良好的回收率(85.0~106.5%)，相对标准偏差(RSD)小于12.1%。开发的方法的成功应用表明，这种整体式吸附剂在有效分离和预富集血样中的痕量分析物方面具有巨大的潜力。(C)2020爱思唯尔B.V.保留所有权利。
In this work, a biocompatible monolithic column based micro-solid-phase extraction (mu-SPE) method was developed for biological fluid analysis. A novel nanoparticle-based polyacrylonitrile monolithic column (C30 NP-PMC) was fabricated by incorporating triacontyl (C30) modified silica nanoparticles (NPs) into the polyacrylonitrile monolithic matrix through thermally induced phase separation. With efficient mass transfer and sorption capacity, C30 NP-PMC exhibited outstanding performance for the extraction of carotenoids and fat-soluble vitamins (FSVs) from human serum samples, superior to commercial C18 cartridges as well as liquid-liquid extraction (LLE) method. Under optimal conditions, the proposed mu-SPE method coupled with high-performance liquid chromatography-diode array detection (HPLC-DAD) achieved satisfactory limits of detection (LODs) (1.5-75.0 ng/mL) and good recoveries (85.0-106.5 %) with relative standard deviations (RSDs) of less than 12.1% by consuming lower sorbent (35.0 mg) and organic solvent (0.8 mL). Successful application of the developed method demonstrated the great potential of such monolithic sorbents for efficient isolation and preconcentration of trace analytes from blood samples. (C) 2020 Elsevier B.V. All rights reserved.