Biocompatibility of resin composites subcutaneously implanted in rats with experimentally induced arthritis
Biocompatibility of resin composites subcutaneously implanted in rats with experimentally induced arthritis
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DOI：
10.1016/j.dental.2008.09.012
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发表时间：
2009-07-01
期刊：
影响因子：
5
通讯作者：
Tesseromatis, C.
中科院分区：
文献类型：
--
作者：
Anagnostou, M.;Chatzigianni, E.;Tesseromatis, C.
客观的。评价不同固化效率的树脂复合材料标本皮下植入实验性关节炎大鼠体内的生物相容性。方法。通过微衰减全反射傅里叶变换红外光谱测量光聚合 10 秒和 40 秒曝光时间 (n = 3) 的杂化树脂复合材料样品的剩余 C=C 键量 (%RDB)。将雄性 Wistar 大鼠 (n = 36) 分为两组 (n = 18)：健康动物和实验诱导关节炎的动物。将照射10秒和40秒的树脂复合材料样本和氢氧化钙对照样本植入每只动物的背部皮下。 2周、4周和9周后处死动物。关节炎的发展是通过生化分析和动物器官（脾、胸腺、肾上腺）相对重量的变化来定义的。对组织反应进行组织学检查。结果。每个位点的 %RDB 和暴露时间显示出统计上显着的差异。暴露 40 秒的样本记录了最低的 %RDB 值。生化指数和相对器官重量显示健康动物和患有关节炎的动物之间存在统计学上的显着差异。动物的健康状况和使用的材料不影响组织反应。与第三阶段相比，第一和第二阶段的处死显示出结缔组织发育的倾向降低。关于巨细胞的存在，这同样适用于第二期。意义。与对照组相比，测试的材料和动物的健康状况并未导致组织反应改变。处死时间与不同的组织反应相关。 (C) 2009 年牙科材料学会。由爱思唯尔有限公司出版。保留所有权利。
Objective. To evaluate the biocompatibility of resin composite specimens with different curing efficiency, subcutaneously implanted in rats with experimentally induced arthritis.Methods. The amount of remaining C=C bonds (%RDB) of hybrid resin composite specimens photopolymerized for 10 s and 40 s exposure time (n = 3) was measured by micro-attenuated total reflectance Fourier transform infrared spectroscopy Male Wistar rats (n = 36) were classified in two groups (n = 18) of healthy animals and of animals with experimentally induced arthritis. Resin composite specimens irradiated for 10 s and 40 s and calcium hydroxide control specimens were implanted subcutaneously in each animals' dorsum. Following 2-, 4- and 9-week periods the animals were sacrificed. The development of arthritis was defined by biochemical analysis and the changes in the relative weight of animals' organs (spleen, thymus, adrenals). Tissue reactions were examined histologically.Results. %RDB per site and exposure time showed statistically significant differences. Lowest %RDB values were recorded on 40 s exposed specimens. Biochemical indices and relative organ weights demonstrated statistically significant differences between healthy animals and animals with arthritis. The health status of the animals and the materials used did not influence tissue response. First and second periods of sacrifice showed reduced propensity of connective tissue development in comparison to the third period. The same applied for the second period regarding the presence of giant cells.Significance. The materials tested and the animals' health status did not result in altered tissue response compared to control group. The period of sacrifice was associated with different tissue responses. (C) 2009 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.