Improved procedures for the determination of lipid phosphorus by malachite green.
Improved procedures for the determination of lipid phosphorus by malachite green.
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DOI：
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发表时间：
1992-08
影响因子：
6.5
通讯作者：
X. Zhou;G. Arthur
中科院分区：
文献类型：
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作者：
X. Zhou;G. Arthur
我们开发了两种基于磷钼和孔雀石绿之间相互作用的脂质磷测量程序。一种方法是“微量”测定，使用 50-200 微升 HClO4，灵敏度范围为 0.01-1.5 微克磷。第二种方法是“宏观”测定，其灵敏度范围为 0.03-5.0 微克磷和 100-500 微升 HClO4。两种测定的重复性都非常好，无论使用的 HClO4 含量如何，三次重复之间的日常标准偏差均小于 6%。在不同浓度的 HClO4 下，每种方法都成功地测定了磷脂酰乙醇胺和鞘磷脂标准品中的磷含量，涵盖了建议的灵敏度范围。程序中允许的范围、灵敏度和 HClO4 体积的增加代表了对现有方法的重大改进。
We have developed two procedures for the measurement of lipid phosphorus based on interaction between phosphomolybdenum and malachite green. One method, the "micro" assay uses 50-200 microliters of HClO4 and has a sensitivity range of 0.01-1.5 micrograms phosphorus. The second method, the "macro" assay, has a sensitivity range of 0.03-5.0 micrograms phosphorus with 100-500 microliters HClO4. Both assays are very reproducible with day to day standard deviations of less than 6% between triplicates irrespective of the HClO4 content used. At different concentrations of HClO4, each method was successfully used to determine the phosphorus content in phosphatidylethanolamine and sphingomyelin standards that covered the proposed sensitivity ranges. The increased range, sensitivity and greater volumes of HClO4 permitted in the procedures represent significant improvements over existing methods.