Simultaneous analysis of flavonoids from Hypericum japonicum Thunb.ex Murray (Hypericaceae) by HPLC-DAD-ESI/MS.
Simultaneous analysis of flavonoids from Hypericum japonicum Thunb.ex Murray (Hypericaceae) by HPLC-DAD-ESI/MS.
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DOI：
10.1016/j.jpba.2007.10.032
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发表时间：
2008-01
影响因子：
3.4
通讯作者：
Juan Su;P. Fu;Yunheng Shen;Chuan Zhang;Ming-jin Liang;Run-hui Liu;Huiliang Li;Weidong Zhang
中科院分区：
文献类型：
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作者：
Juan Su;P. Fu;Yunheng Shen;Chuan Zhang;Ming-jin Liang;Run-hui Liu;Huiliang Li;Weidong Zhang
开发了一种新颖、灵敏的 HPLC-UV 方法，用于同时测定金丝桃水醇提取物中的五种主要黄酮类化合物。使用高效液相色谱-二极管阵列检测器与电喷雾串联质谱仪联用，获得了五种黄酮类化合物的化学特征，包括紫杉素-7-O-α-l-鼠李糖苷 (1)、异槲皮苷 (2)、槲皮素 (3)、槲皮素-7-O-α-l-鼠李糖苷 (4) 和槲皮素 (5) （HPLC-DAD-ESI/MS）。分析采用 ZORBAX SB-C18 分析柱（5μm，250mm×4.6mm，内径），梯度溶剂系统为乙腈-0.5% 甲酸水溶液。进行了验证，线性（r2>0.9997）和回收率（范围从 98.4% 到 99.8%）是可以接受的。这些类黄酮的检测限 (LOD) 范围为 0.5 至 7.5 ng。结果表明，不同产地的刺参中黄酮类化合物含量差异较大，含量范围为2.00～34.18mg/g。该方法简单、有效，适用于该中药的质量控制。
A novel and sensitive HPLC-UV method has been developed for the simultaneous determination of five major flavonoids in Hypericum japonicum hydroalcoholic extract. The chemical profile of five flavonoids, including taxfolin-7-O-α-l-rhamnoside (1), isoquercitrin (2), quercitrin (3), quercetin-7-O-α-l-rhamnoside (4) and quercetin (5) was acquired by using high-performance liquid chromatography-diode array detector coupled to an electrospray tandem mass spectrometer (HPLC-DAD–ESI/MS). The analysis was performed on a ZORBAX SB-C18 analytical column (5μm, 250mm×4.6mm, i.d.) with a gradient solvent system of acetonitrile-0.5% aqueous formic acid. The validation was carried out and the linearities (r2>0.9997) and recoveries (ranged from 98.4% to 99.8%) were acceptable. The limits of detection (LOD) of these flavonoids ranged from 0.5 to 7.5ng. The results indicated that the contents of investigated flavonoids in H. japonicum varied significantly from habitat to habitat with contents ranging from 2.00 to 34.18mg/g. The proposed method is simple, effective and suitable for the quality control of this traditional Chinese medicine (TCM).