MOLECULAR EVIDENCE FOR NATURALLY-OCCURRING SINGLE VP7 GENE SUBSTITUTION REASSORTANT BETWEEN HUMAN ROTAVIRUSES BELONGING TO 2 DIFFERENT GENOGROUPS
MOLECULAR EVIDENCE FOR NATURALLY-OCCURRING SINGLE VP7 GENE SUBSTITUTION REASSORTANT BETWEEN HUMAN ROTAVIRUSES BELONGING TO 2 DIFFERENT GENOGROUPS
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DOI：
10.1007/bf01314324
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发表时间：
1991-01-01
影响因子：
2.7
通讯作者：
NAKAGOMI, T
中科院分区：
文献类型：
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作者：
NAKAGOMI, O;NAKAGOMI, T
从8年期间收集的腹泻标本中选择24种包含22种不同电泳型的粪便轮状病毒进行基因组分析。这22种电泳型占先前研究中确定的总电泳型的46%，占收集的328份（64%）轮状病毒标本。将这些粪便轮状病毒的基因组rna与代表三种人类轮状病毒基因群Wa、DS-1和u -1的原型制备的p -32标记的转录探针杂交后，任何一株分离物仅与三种探针中的一种具有高度同源性，这一数据证实并扩展了我们之前关于人类轮状病毒中存在三种不同基因群的观察结果。然而，两种具有血清型（G1）、亚群(I)和RNA模式（相同的短电泳型）的不寻常组合的粪便轮状病毒产生了表明基因群间单一VP7基因替代重组的杂交模式。经细胞培养和RNA-RNA杂交分析，得到VP7基因来源于Wa基因组的分子证据，其余10个基因来源于DS-1基因组的病毒。有趣的是，这些自然重组出现在G1毒株占主导地位的轮状病毒季节中。
Twenty four stool rotaviruses that comprised 22 distinct electropherotypes were selected for genome analysis from the collection of diarrheal specimens obtained over an eight-year period. These 22 electropherotypes were found in 46% of the total electropherotypes identified during the previous studies and represented 328 (64%) of rotavirus specimens in the collection. When genomic RNAs from these stool rotaviruses were hybridized to the P-32-labelled transcription probes prepared from prototypes representing three human rotavirus genogroups, Wa, DS-1, and AU-1, any one of the isolates showed a high degree of homology only with one of the three probes, which data confirmed and extended our previous observation on the existence of three distinct genogroups among human rotaviruses. Two stool rotaviruses which had an unusual combination of serotype (G1), subgroup (I) and RNA pattern (an identical short electropherotype), however, yielded the hybridization pattern indicative of an intergenogroupic single VP7 gene substitution reassortant. When they were cell culture adapted and analyzed by RNA-RNA hybridization, molecular evidence was obtained indicating that their VP7 gene derived from viruses belonging to the Wa genogroup whereas the remaining 10 genes hybridized with viruses belonging to the DS-1 genogroup. Interestingly, these natural reassortants emerged in the midst of the rotavirus season in which G1 strains predominated.