CHARACTERISTICS OF FELINE PANLEUCOPAENIA VIRUS STRAINS ENABLING DEFINITIVE CLASSIFICATION AS PARVOVIRUSES
CHARACTERISTICS OF FELINE PANLEUCOPAENIA VIRUS STRAINS ENABLING DEFINITIVE CLASSIFICATION AS PARVOVIRUSES
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DOI：
10.1007/bf01240073
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发表时间：
1974-01-01
期刊：
影响因子：
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通讯作者：
GAUTSCHI, M
中科院分区：
文献类型：
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作者：
JOHNSON, RH;SIEGL, G;GAUTSCHI, M
引起猫泛白细胞减少症和水貂肠炎的病毒显示具有细小病毒的形态，平均直径在21和24 nm之间。浮力密度研究表明，两种毒株的纯化病毒均由主要密度为1.41 g/ml的“完整”病毒带的颗粒组成，少数群体密度为1.44 g/ml。其他DNA含量较低或检测不到DNA的颗粒种类分别出现在平均浮力密度为1.36和1.31 g/ml的情况下。对于猫泛白细胞减少症和水貂肠炎病毒，含有DNA的三个密度水平的DNA沉降系数给出的结果与单链DNA的预期结果一致，而浮力密度研究表明，所有三个物种的DNA平均密度为1.722。在1.41 g/ml处条带化的颗粒的DNA的计算分子量（1.7× 106道尔顿）占总颗粒重量的28.5%（5.9× 106道尔顿）。病毒蛋白可分为两种主要多肽，分子量分别为60，300和73，100，分别占86%和10%左右，放射性氨基酸的总量。分子量为39 600道尔顿的第三种次要成分的浓度在3%至6%之间。
The viruses causing feline panleucopaenia and mink enteritis are shown to have the morphology of parvoviruses, with a mean diameter between 21 and 24 nm. Buoyant density studies show that purified virus of both strains consists of particles which band as “complete” virus at a main density of 1.41 g/ml, with a minor population at a density of 1.44 g/ml. Other particle species with a lower content of DNA, or no detectable DNA, occurred at mean buoyant densities of 1.36 and 1.31 g/ml, respectively.For both feline panleucopaenia and mink enteritis viruses, sedimentation coefficients of DNA from the three density levels which contain DNA, gave results conforming to those expected for single stranded DNA, whilst buoyant density studies showed the DNA of all three species to have a mean density of 1.722. The calculated molecular weight of the DNA of particles banding at 1.41 g/ml (1.7×106daltons) represents 28.5 per cent of the total particle weight (5.9×106daltons).Viral protein can be separated into two major polypeptides with molecular weights of 60,300 and 73,100, amounting to 86 and about 10 per cent, respectively, of the total radioactive amino acids incorporated. A third minor component of molecular weight 39,600 daltons was present at concentrations between 3 and 6 per cent.