Vacuum/Compression Infiltration-mediated Permeation Pathway of a Peptide-pDNA Complex as a Non-Viral Carrier for Gene Delivery in Planta
Vacuum/Compression Infiltration-mediated Permeation Pathway of a Peptide-pDNA Complex as a Non-Viral Carrier for Gene Delivery in Planta
复制标题
DOI：
10.1038/s41598-018-36466-1
复制
发表时间：
2019-01-22
影响因子：
4.6
通讯作者：
Numata, Keiji
中科院分区：
文献类型：
--
作者：
Midorikawa, Keiko;Kodama, Yutaka;Numata, Keiji
非病毒基因载体已被广泛研究，作为替代病毒载体的基因递送系统进入动物和植物细胞。先前开发了含有细胞穿透肽和阳离子序列的非病毒基因载体用于完整植物和植物细胞;然而，基因载体进入植物细胞的渗透途径尚未阐明，这将有助于提高基因递送效率。在这里，我们确定了真空/压缩渗透介导的渗透途径的非病毒基因载体进入植物组织和细胞，使用复合体的质粒DNA和基于肽的基因载体。该复合物在拟南芥中通过排水管吸收，在本氏烟草中从根毛中吸收。值得注意的是，这些结构脆弱的组织也是自然界中细菌入侵的途径，这表明肽-pDNA复合物通过与细菌病原体相似的途径入侵完整植物。
Non-viral gene carriers have been extensively investigated as alternatives to viral vectors for gene delivery systems into animal and plant cells. A non-viral gene carrier containing a cell-penetrating peptide and a cationic sequence was previously developed for use in intact plants and plant cells; however, the permeation pathway of the gene carrier into plant cells is yet to be elucidated, which would facilitate the improvement of the gene delivery efficiency. Here, we identified the vacuum/compression infiltration-mediated permeation pathway of a non-viral gene carrier into plant tissues and cells using a complex of plasmid DNA and a peptide-based gene carrier. This complex was taken up via the hydathodes in Arabidopsis thaliana, and from root hairs in Nicotiana benthamiana. Remarkably, these structurally weak tissues are also routes of bacterial invasion in nature, suggesting that peptide-pDNA complexes invade intact plants through similar pathways as bacterial pathogens.