Characterization and functional analysis of a cAMP-dependent protein kinase A catalytic subunit gene (pka1) in Sclerotinia sclerotiorum
Characterization and functional analysis of a cAMP-dependent protein kinase A catalytic subunit gene (pka1) in Sclerotinia sclerotiorum
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DOI：
10.1016/j.pmpp.2004.07.004
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发表时间：
2004-03-01
影响因子：
2.7
通讯作者：
Rollins, JA
中科院分区：
文献类型：
--
作者：
Jurick, WM;Dickman, MB;Rollins, JA
cAMP 水平升高会抑制核盘菌的菌核发育并增加草酸盐的产生。假设这些作用是由蛋白激酶 A (PKA) 介导的。为了检验这一假设，克隆并破坏了 pka1 催化亚基基因。 Southern 杂交证实了破坏，Northern 分析显示野生型 pka1 转录本丢失。突变株具有 cAMP 响应性、致病性，并具有野生型水平的 PKA 活性。 pka1 与其他真菌 pka 基因和编码肽的系统发育分析表明丝状真菌拥有两个 pka 旁系同源物。这项工作中提出的多项证据表明，核盘菌中存在第二个 pka 催化亚基基因 pka2，并贡献了大部分 PKA 活性。 (C) 2004 Elsevier Ltd. 保留所有权利。
Increased cAMP levels inhibit sclerotial development and raise oxalate production in Sclerotinia sclerotiorum. These effects were hypothesized to be mediated by protein kinase A (PKA). To test this hypothesis, a pka1 catalytic subunit gene was cloned and disrupted. Southern hybridization confirmed disruption, and northern analysis revealed loss of a wild-type pka1 transcript. Mutant strains were cAMP-responsive, pathogenic, and had wild-type levels of PKA activity. Phylogenetic analyses of pka1 with other fungal pka genes and encoded peptides suggest that filamentous fungi possess two pka paralogs. Multiple lines of evidence presented in this work suggest that a second pka catalytic subunit gene, pka2, exists and contributes the majority of PKA activity in S. sclerotiorum. (C) 2004 Elsevier Ltd. All rights reserved.