Diagnostic value of urinary CXCL10 as a biomarker for predicting Hunner type interstitial cystitis
Diagnostic value of urinary CXCL10 as a biomarker for predicting Hunner type interstitial cystitis
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DOI：
10.1002/nau.23431
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发表时间：
2018-03-01
影响因子：
2
通讯作者：
Homma, Yukio
中科院分区：
文献类型：
--
作者：
Niimi, Aya;Igawa, Yasuhiko;Homma, Yukio
目的探讨Hunner型间质性膀胱炎（HIC）患者膀胱组织中趋化因子和细胞因子水平作为鉴别HIC与非Hunner型间质性膀胱炎（NHIC）的尿标志物的可行性。对尿液样本的上清液进行ELISA试剂盒以测量10种细胞因子和趋化因子，已知其基因表达在HIC膀胱组织中升高。比较三组间尿肌酐（Cr）浓度标准化的尿水平。通过ROC分析探讨IC和IC亚型的区分效率。标记物水平与症状严重程度的相关性，评估O 'Leary-Sant的症状指数（OSSI）和问题指数（OSPI），examined.ResultsThe尿水平的CXCL 10和NGF显着高于HIC比NHIC。CXCL 10和NGF区分HIC和NHIC的AUC分别为0.78和0.68。CXCL 10和NGF水平的组合产生0.81的AUS。CXCL 10临界值为53.2pg/mg Cr时，敏感性为46.1%，特异性为93.7%，阳性预测值为97.7%，阴性预测值为60.0%。尿中其他细胞因子水平在HIC和NHIC之间无显著性差异。结论尿CXCL 10水平升高与NGF水平升高联合或不联合均可作为鉴别HIC和NHIC的辅助生物标志物，其敏感性适中，特异性较高。
AimTo investigate the feasibility of chemokines and cytokines potentially elevated in the bladder tissue of Hunner type interstitial cystitis (HIC) as urinary markers for distinguishing HIC from non-Hunner type interstitial cystitis (NHIC)MethodsUrine specimens were collected from 41 HIC patients, 25 NHIC patients, and 31 healthy volunteers (control). The supernatants of urine specimens were subjected to ELISA kits for measurements of 10 cytokines and chemokines, whose gene expression was known to be elevated in HIC bladder tissue. Urinary levels normalized by urinary creatinine (Cr) concentration were compared among three groups. Efficiency in differentiating IC and IC subtypes was explored by ROC analysis. The correlation of marker levels with symptom severity, assessed by O'Leary-Sant's symptom index (OSSI) and problem index (OSPI), was examined.ResultsThe urinary levels of CXCL10 and NGF were significantly higher in HIC than NHIC. CXCL10 and NGF differentiated HIC against NHIC with AUC of 0.78 and 0.68, respectively. Combination of CXCL10 and NGF levels yielded an AUS of 0.81. The CXCL10 cut-off of 53.2pg/mg Cr had sensitivity of 46.1%, specificity of 93.7%, positive predictive value of 97.7%, and negative predictive value of 60.0%. The urinary level of other cytokines showed no significant difference between HIC and NHIC. Significant correlation with symptoms was detected for CXCL10 alone.ConclusionThe results suggested that increased urinary level of CXCL10 combined with or without high NGF level could be a promising supplementary biomarker for differentiating HIC from NHIC with modest sensitivity and high specificity.