Bombyx mori protein BmREEPa and BmPtchd could form a complex with BmNPV envelope protein GP64.
Bombyx mori protein BmREEPa and BmPtchd could form a complex with BmNPV envelope protein GP64.
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DOI：
10.1016/j.bbrc.2017.07.004
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发表时间：
2017-09
影响因子：
3.1
通讯作者：
Xiao-long Dong;Yun-fei Wu;Tai-Hang Liu;Wei Wang;C. Pan;M. Adur;Min-juan Zhang;M. Pan;Cheng Lu
中科院分区：
文献类型：
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作者：
Xiao-long Dong;Yun-fei Wu;Tai-Hang Liu;Wei Wang;C. Pan;M. Adur;Min-juan Zhang;M. Pan;Cheng Lu
我们前期的研究表明，BmREEPa在体内外均参与BV的侵袭，但其机制尚不清楚。本研究通过免疫共沉淀法筛选了BmREEPa相互作用蛋白，并最终鉴定出一个具有受体活性的膜蛋白--BmPtchd，KR 338939。进一步的研究表明BmPtchd、BmREEPa和Glycoprotein 64可以形成蛋白复合物，BmREEPa和BmPtchd的表达水平受细胞胆固醇水平的影响。这些结果可能为解释家蚕核型多角体病毒出芽病毒的入侵机制提供重要依据。
Our previous studies have indicated thatBombyx morireceptor expression enhancing protein a (BmREEPa) could participate in BV invasionin vivoandin vitro, however, the mechanism is still unclear. In this study, we screened BmREEPa interacting protein through co-immunoprecipitation and finally identified a membrane protein,Bombyx moripatched domain containing protein (BmPtchd, KR338939), which contains receptor activity. Further studies showed that BmPtchd, BmREEPa and Glycoprotein 64 could form a protein complex and the expression level of BmREEPa and BmPtchd could be affected by cellular cholesterol level. These findings may provide an important basis for explaining the invasion mechanism ofBombyx moriNucleopolyhedrovirus budded virus.