Role of modulator recognition factor 2 in adipogenesis and leptin expression in 3T3-L1 cells.
Role of modulator recognition factor 2 in adipogenesis and leptin expression in 3T3-L1 cells.
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DOI：
10.1016/j.bbrc.2007.12.002
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发表时间：
2008-02
影响因子：
3.1
通讯作者：
Jie Dong;Naoki Ishimori;B. Paigen;H. Tsutsui;S. Fujii
中科院分区：
文献类型：
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作者：
Jie Dong;Naoki Ishimori;B. Paigen;H. Tsutsui;S. Fujii
脂肪形成转录因子的复杂网络调节脂肪细胞分化、肥胖和胰岛素抵抗。调节器识别因子 (Mrf) 2 敲除小鼠表现出脂肪积累缺陷，并且不会受到饮食诱导的肥胖的影响，这表明 Mrf2 缺陷会影响脂肪生成。在此，我们报道转录因子 Mrf2、Mrf2α 和 Mrf2β 的 2 种亚型的基因表达在 3T3-L1 细胞的脂肪生成过程中被诱导。 3T3-L1 前脂肪细胞和分化脂肪细胞中 Mrf2 mRNA 表达对胰岛素、地塞米松和 TNF-α 的刺激敏感。小干扰RNA诱导的Mrf2α和Mrf2β基因表达下调增加了瘦素的mRNA表达。这些结果表明Mrf2可能是脂肪细胞分化的潜在调节剂和瘦素的潜在阻遏物。
The complex network of adipogenic transcription factors regulates adipocyte differentiation, obesity, and insulin resistance. Modulator recognition factor (Mrf) 2 knockout mice exhibit defects in fat accumulation and are protected from diet-induced obesity, suggesting that Mrf2 deficiency affects adipogenesis. Here, we report that the gene expressions of the 2 isoforms of the transcription factors Mrf2, Mrf2α, and Mrf2β, were induced upon adipogenesis in 3T3-L1 cells. Mrf2 mRNA expression was sensitive to stimulation by insulin, dexamethasone, and TNF-α in 3T3-L1 preadipocytes and differentiated adipocytes. Down-regulation of Mrf2α and Mrf2β gene expressions induced by small interfering RNAs increased the mRNA expression of leptin. These results indicate that Mrf2 can be a potential regulator of adipocyte differentiation and a potential repressor of leptin.