Abdominal and Pelvic Organ Failure Induced by Intraperitoneal Influenza A Virus Infection in Mice
Abdominal and Pelvic Organ Failure Induced by Intraperitoneal Influenza A Virus Infection in Mice
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DOI：
10.3389/fmicb.2020.01713
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发表时间：
2020-07-17
影响因子：
5.2
通讯作者：
Kwon, Hyung-Joo
中科院分区：
文献类型：
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作者：
Gautam, Avishekh;Akauliya, Madhav;Kwon, Hyung-Joo
在人类中，甲型流感病毒的呼吸道感染可能是致命的，但目前尚不清楚非呼吸道甲型流感感染是否也同样致命。腹膜内感染使腹腔和盆腔器官容易受到病原体的感染，因为腹膜液在整个盆腔和腹部循环。我们发现，用甲型流感病毒对小鼠进行高剂量腹膜内感染，导致胰腺严重硬化和结构损伤，卵巢卵泡破裂，子宫中免疫细胞大量浸润。腹膜内感染还引起促炎介质（包括IL-6、BLC和IL-4）的强烈上调。此外，一种流感病毒株的低剂量腹腔内感染提供了对随后另一种流感病毒株的腹腔内或鼻内攻击的交叉保护。我们的研究结果表明，低剂量，非呼吸道给药可能提供了一种流感疫苗接种途径。此外，这些结果提供了关于甲型流感病毒在高危患者（包括女性和糖尿病患者）中的病理作用的见解。
In humans, respiratory infections with influenza A viruses can be lethal, but it is unclear whether non-respiratory influenza A infections can be equally lethal. Intraperitoneal infection makes the abdominal and pelvic organs accessible to pathogens because of the circulation of peritoneal fluid throughout the pelvis and abdomen. We found that high-dose intraperitoneal infection in mice with influenza A viruses resulted in severe sclerosis and structural damage in the pancreas, disruption of ovarian follicles, and massive infiltration of immune cells in the uterus. The intraperitoneal infections also caused robust upregulation of proinflammatory mediators including IL-6, BLC, and MIG. In addition, low-dose intraperitoneal infection with one influenza strain provided cross-protection against subsequent intraperitoneal or intranasal challenge with another influenza strain. Our results suggest that low-dose, non-respiratory administration might provide a route for influenza vaccination. Furthermore, these results provide insight on the pathological role of influenza A viruses in high-risk patients, including women and diabetic individuals.