Phenotypic expression of Hodgkin's and Reed-Sternberg cells in Hodgkin's disease.
Phenotypic expression of Hodgkin's and Reed-Sternberg cells in Hodgkin's disease.
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霍奇金病和里德-斯滕伯格细胞在霍奇金病中的表型表达。
作者：
S. Hsu;K. Yang;E. Jaffe
通过免疫组织化学技术用一组单克隆抗体和花生凝集素（PNA）分析确定霍奇金和里德-斯滕伯格（H-RS）细胞的表型表达。其中T200、抗HLA-DR、抗Leu 10、A1 G3、抗Tac、OKT 9和抗Leu M1 7种抗体与绝大多数H-RS细胞反应。在某些情况下，H-RS细胞也结合PNA。其他抗体，包括对T细胞（如Lyt 3）和B细胞（如B1，抗Leu 14）具有高度特异性的抗体，均为阴性。这些结果反对H-RS细胞来源于T或B淋巴细胞。H-RS细胞也用与单核细胞（OKM 1、Mo-2、63 D-3）、滤泡树突细胞（DRC-1）和自然杀伤/杀伤细胞（Leu 7、Leu 11 a、B73.1）反应的抗体负染色。H-RS细胞中Leu M1和Tac的存在是令人感兴趣的。抗Leu M1阳性被认为是在所有20个霍奇金病（HD）的情况下进行测试，并应提供一个非常有用的试剂，从其他反应性和肿瘤性疾病的HD的鉴别诊断。Tac通常只存在于活化的T细胞上。H-RS细胞中Tac的存在可能反映了肿瘤转化阶段T细胞生长因子受体或密切相关蛋白的表达。虽然这些研究不能确定HD肿瘤细胞的性质，但它们与交错网状细胞的起源一致。H-RS细胞和交错网状细胞具有相似的抗原表型（Leu M1+、T200+、HLA-DR+、Leu 10+、A1 G3+和OKT 9+）和相似的溶酶体酶活性模式。
The phenotypic expression of Hodgkin's and Reed-Sternberg (H-RS) cells was determined by analysis with a panel of monoclonal antibodies and peanut agglutinin (PNA) by an immunohistochemical technique. Seven antibodies, including T200, anti-HLA-DR, anti-Leu 10, A1G3, anti-Tac, OKT9, and anti-Leu M1, were found to react with a great majority of H-RS cells. In some cases, H-RS cells also bound PNA. Other antibodies, including those highly specific for T cells (eg, Lyt 3) and B cells (eg, B1, anti-Leu 14) were consistently negative. The results argue against the derivation of H-RS cells from T or B lymphocytes. The H-RS cells were also negatively stained with antibodies which react with monocytes (OKM1, Mo-2, 63D-3), follicular dendritic cells (DRC-1), and natural killer/killer cells (Leu 7, Leu 11a, B73.1). The presence of Leu M1 and Tac in H-RS cells is of interest. Anti-Leu M1 positivity was seen in all 20 of Hodgkin's disease (HD) cases tested and should provide a very useful reagent for differential diagnosis of HD from other reactive and neoplastic conditions. Tac normally is present only on activated T cells. The presence of Tac in H-RS cells may reflect expression of T-cell growth factor receptor or a closely related protein during a stage of neoplastic transformation. Although the nature of the neoplastic cell of HD cannot be determined by these studies, they are consistent with an origin from interdigitating reticulum cells. Both H-RS cells and interdigitating reticulum cells have a similar antigenic phenotype (Leu M1+, T200+, HLA-DR+, Leu 10+, A1G3+, and OKT9+) and a similar pattern of lysosomal enzyme activity.
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霍奇金病的组织化学。
DOI：
--
发表时间：
1982
期刊：
Cancer treatment reports
影响因子：
--
作者：
Beckstead,JH;Warnke,R;Bainton,DF
通讯作者：
Bainton,DF
霍奇金病中的淋巴结 T 细胞：用单克隆抗体和玫瑰花结技术分析悬浮液。
影响因子：
20.3
作者：
Aisenberg,AC;Wilkes,BM
通讯作者：
Wilkes,BM
霍奇金病、非霍奇金淋巴瘤和反应性增生中含有胞壁质酶的细胞的发生和模式。
影响因子：
3.3
作者：
Ree,HJ;Song,JY;Leone,LA;Crowley,JP;Fanger,H
通讯作者：
Fanger,H
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Haynes,BF;Harden,EA;Telen,MJ;Hemler,ME;Strominger,JL;Palker,TJ;Scearce,RM;Eisenbarth,GS
通讯作者：
Eisenbarth,GS
鲎木林的纯化和用途：唾液酸特异性凝集素。
DOI：
10.1177/30.9.6897073
发表时间：
1982
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Muresan,V;Iwanij,V;Smith,ZD;Jamieson,JD
通讯作者：
Jamieson,JD