Genotyping Genome-Edited Founders and Subsequent Generation.
Genotyping Genome-Edited Founders and Subsequent Generation.
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基因组编辑创始人和后代的基因分型。
DOI：
10.1007/978-1-0716-2990-1_4
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Mackenzie M
中科院分区：
文献类型：
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作者：
Mackenzie M
靶向核酸酶允许直接在早期胚胎中产生多种类型的基因突变。然而，它们活动的结果是不可预测的修复事件，并且产生的始祖动物通常具有马赛克性质。在这里，我们提出了分子测定和基因分型策略，这些策略将支持根据产生的突变类型，筛选第一代的潜在创始人，并验证下一代的阳性动物。
Targeted nucleases allow the production of many types of genetic mutations directly in the early embryo. However, the outcome of their activity is a repair event of unpredictable nature, and the founder animals that are produced are generally of a mosaic nature. Here, we present the molecular assays and genotyping strategies that will support the screening of the first generation for potential founders and the validation of positive animals in the subsequent generation, according to the type of mutation generated.