The Met proto-oncogene mesenchymal to epithelial cell conversion.
The Met proto-oncogene mesenchymal to epithelial cell conversion.
复制标题
DOI：
10.1126/science.7505952
复制
发表时间：
1994-01
期刊：
影响因子：
56.9
通讯作者：
I. Tsarfaty;S. Rong;J. Resau;S. Rulong;P. P. D. Silva-P.;G. Woude
中科院分区：
文献类型：
--
作者：
I. Tsarfaty;S. Rong;J. Resau;S. Rulong;P. P. D. Silva-P.;G. Woude
共表达的人甲硫氨酸受体及其配体，肝细胞生长因子/分散因子（HGF/SF），在NIH 3 T3成纤维细胞导致细胞成为裸鼠致瘤性。所产生的肿瘤显示管腔样形态，含有癌样病灶区，细胞间连接类似桥粒，共表达上皮（细胞角蛋白）和间充质（波形蛋白）细胞骨架标记物。肿瘤细胞还显示桥粒和紧密连接蛋白的表达增强。肿瘤细胞的表观间充质至上皮转化模拟胚胎肾发育期间发生的转化，表明Met-HGF/SF信号传导在该过程中以及在表达上皮和间充质标志物的肿瘤中起作用。
Coexpression of the human Met receptor and its ligand, hepatocyte growth factor/scatter factor (HGF/SF), in NIH 3T3 fibroblasts causes the cells to become tumorigenic in nude mice. The resultant tumors display lumen-like morphology, contain carcinoma-like focal areas with intercellular junctions resembling desmosomes, and coexpress epithelial (cytokeratin) and mesenchymal (vimentin) cytoskeletal markers. The tumor cells also display enhanced expression of desmosomal and tight-junction proteins. The apparent mesenchymal to epithelial conversion of the tumor cells mimics the conversion that occurs during embryonic kidney development, suggesting that Met-HGF/SF signaling plays a role in this process as well as in tumors that express both epithelial and mesenchymal markers.