Reduced MLH3 Expression in the Syndrome of Gan-Shen Yin Deficiency in Patients with Different Diseases
Reduced MLH3 Expression in the Syndrome of Gan-Shen Yin Deficiency in Patients with Different Diseases
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DOI：
10.1155/2017/4109828
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发表时间：
2017-09
期刊：
影响因子：
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通讯作者：
Juan Du;Maofeng Zhong;Dong-bing Liu;Shufang Liang;Xiao-Lin Liu;Binbin Cheng;Ya'ni Zhang;Zi-fei Yin;Yuan Wang;C. Ling
中科院分区：
文献类型：
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作者：
Juan Du;Maofeng Zhong;Dong-bing Liu;Shufang Liang;Xiao-Lin Liu;Binbin Cheng;Ya'ni Zhang;Zi-fei Yin;Yuan Wang;C. Ling
中医根据体质辨证施治。不同体质具有特定的代谢特点，对某些疾病的易感性也不同。本研究旨在探讨不同疾病中肝肾阴虚体质的特征基因。选取原发性肝癌（PLC）患者50例、高血压（HBP）患者94例、糖尿病（DM）患者100例，根据体质调查表分为肝肾阴虚组和非肝肾阴虚组，评价患者的临床表现。肝肾阴虚证患者与非肝肾阴虚证患者有17个基因的mRNA表达差异。但在所有的原发性肝癌、高血压和糖尿病患者中，只有肝肾阴虚组的MLH 3显著低于非肝肾阴虚组。经ROC曲线分析，证实MLH 3与肝肾阴虚体质之间存在相关性。用阴虚经方六味地黄丸治疗MLH 3（-/-和-/+）小鼠，可部分改善MLH 3（-/+）小鼠的阴虚体质，但对MLH 3（-/-）小鼠无改善作用。MLH 3可能是肝肾阴虚体质的物质基础之一。
Traditional Chinese medicine formulates treatment according to body constitution (BC) differentiation. Different constitutions have specific metabolic characteristics and different susceptibility to certain diseases. This study aimed to assess the characteristic genes of gan-shen Yin deficiency constitution in different diseases. Fifty primary liver cancer (PLC) patients, 94 hypertension (HBP) patients, and 100 diabetes mellitus (DM) patients were enrolled and classified into gan-shen Yin deficiency group and non-gan-shen Yin deficiency group according to the body constitution questionnaire to assess the clinical manifestation of patients. The mRNA expressions of 17 genes in PLC patients with gan-shen Yin deficiency were different from those without gan-shen Yin deficiency. However, considering all patients with PLC, HBP, and DM, only MLH3 was significantly lower in gan-shen Yin deficiency group than that in non-gen-shen Yin deficiency. By ROC analysis, the relationship between MLH3 and gan-shen Yin deficiency constitution was confirmed. Treatment of MLH3 (−/− and −/+) mice with Liuweidihuang wan, classical prescriptions for Yin deficiency, partly ameliorates the body constitution of Yin deficiency in MLH3 (−/+) mice, but not in MLH3 (−/−) mice. MLH3 might be one of material bases of gan-shen Yin deficiency constitution.