Immunoreactivity of a Monoclonal Antibody to SS18-SSX Fusion Gene Product in Formalin-fixed Paraffin-embedded Synovial Sarcoma Tissue Section.
Immunoreactivity of a Monoclonal Antibody to SS18-SSX Fusion Gene Product in Formalin-fixed Paraffin-embedded Synovial Sarcoma Tissue Section.
复制标题
单克隆抗体对福尔马林固定石蜡包埋滑膜肉瘤组织切片中 SS18-SSX 融合基因产物的免疫反应性。
DOI：
10.1097/pai.0000000000000413
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Takeuchi T.
中科院分区：
文献类型：
--
作者：
Saigo C;Kito Y;Takeuchi T.
滑膜肉瘤是一种侵袭性肉瘤，具有SS 18（也称为SYT）和SSX基因的特异性相互染色体易位。在本研究中，我们的目的是检测SS 18-SSX融合基因产物在常规处理的病理滑膜肉瘤组织切片。肽QRPYGYDQ-IMPKKPA，其中涵盖了SS 18-SSX的融合区域，新建立的单克隆抗体，随后通过酶联免疫吸附试验，Western免疫印迹，免疫组化染色的特点。命名为BG 35的单克隆抗体，当QRPYGYDQ-IMPKKPA的C-末端或邻近区域结构暴露时，以特异性方式与SS 18-SSX嵌合蛋白的边界区域反应。BG 35的免疫反应定位于滑膜肉瘤细胞的细胞核，而不是在其他肉瘤细胞，检查。有趣的是，滑膜肉瘤细胞与上皮分化表现出更强的BG 35免疫反应性比滑膜肉瘤细胞肉瘤分化。BG 35可用于原位评价SSX-SS 18基因产物的分子动力学。
Synovial sarcoma is an aggressive sarcoma with specific reciprocal chromosomal translocation of SS18 (also known as SYT) and SSX genes. In the present study, we aimed to detect the SS18-SSX fusion gene product in routinely processed pathologic synovial sarcoma tissue section. Monoclonal antibodies to peptide QRPYGYDQ-IMPKKPA, which covered the fusion region of SS18-SSX, were newly established and subsequently characterized by enzyme-linked immunosorbent assay, Western-immunoblotting, and immunohistochemical staining. A monoclonal antibody designated BG35, reacted with boundary region of SS18-SSX chimera protein in a specific manner, when C-terminal or neighboring region of QRPYGYDQ-IMPKKPA is structurally exposed. Immunoreactivity of BG35 was localized in nucleus of synovial sarcoma cells, but not in other sarcoma cells, examined. Interestingly, synovial sarcoma cells with epithelial differentiation exhibited much strong BG35 immunoreactivity than synovial sarcoma cells with sarcomatous differentiation. BG35 may be useful for evaluating molecular kinetics of SSX-SS18 gene product in situ.