Cell Wall Free Space of Cucumis Hypocotyls Contains NAD and a Blue Light-Regulated Peroxidase Activity.
Cell Wall Free Space of Cucumis Hypocotyls Contains NAD and a Blue Light-Regulated Peroxidase Activity.
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黄瓜下胚轴的细胞壁自由空间含有 NAD 和蓝光调节的过氧化物酶活性。
DOI：
10.1104/pp.98.4.1336
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发表时间：
1992
期刊：
影响因子：
7.4
通讯作者：
R. L. Jones
中科院分区：
文献类型：
--
作者：
J. Shinkle;S. Swoap;P. Simon;R. L. Jones
从红光生长的黄瓜（Cucumis sativus L.）下胚轴切片通过低速离心技术。离心液含有NAD和过氧化物酶，但没有可检测的细胞质污染，如细胞壁溶液中葡萄糖-6-磷酸脱氢酶活性的缺乏所示。从红光培养的黄瓜下胚轴切片的细胞壁中离心得到的过氧化物酶活性，经等电聚焦电泳，至少可分为三种阴极同工酶和两种阳极同工酶。用10分钟的高强度蓝光脉冲处理红光生长的黄瓜幼苗，使细胞壁过氧化物酶的水平增加约60%，并引起这种酶的阳极亚型的质的变化。过氧化物酶活性的增加在蓝光脉冲开始后25分钟内可检测到，在35分钟时达到最大，并在照射45分钟时下降至对照水平。蓝光对下胚轴伸长的抑制作用比蓝光对总壁过氧化物酶活性的影响更快，从而得出生长和过氧化物酶活性没有因果关系的结论。
Solutions were obtained from the cell wall free space of red light-grown cucumber (Cucumis sativus L.) hypocotyl sections by a low-speed centrifugation technique. The centrifugate contained NAD and peroxidase but no detectable cytoplasmic contamination, as indicated by the absence of the activity of glucose-6-phosphate dehydrogenase from the cell wall solution. Peroxidase activity centrifuged from the cell wall of red light-grown cucumber hypocotyl section could be resolved into at least three cathodic isoforms and two anodic isoforms by isoelectric focusing. Treatment of red light-grown cucumber seedlings with a 10-minute pulse of high-intensity blue light increased the level of cell wall peroxidase by about 60% and caused a qualitative change in the anodic isoforms of this enzyme. The increase in peroxidase activity was detectable within 25 minutes after the start of the blue light pulse, was maximal at 35 minutes, and declined to control levels by 45 minutes of irradiation. The inhibitory effect of blue light on hypocotyl elongation was more rapid than the effect of blue light on total wall peroxidase activity, leading to the conclusion that growth and peroxidase activity are not causally related.