Glycopolymer Microarrays with Sub‐Femtomolar Avidity for Glycan Binding Proteins Prepared by Grafted‐To/Grafted‐From Photopolymerizations
Glycopolymer Microarrays with Sub‐Femtomolar Avidity for Glycan Binding Proteins Prepared by Grafted‐To/Grafted‐From Photopolymerizations
复制标题
通过 Grafted–To/Grafted–From 光聚合制备的糖聚合物微阵列，对聚糖结合蛋白具有亚飞摩尔亲和力
DOI：
10.1002/anie.202105729
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Braunschweig, Adam B.
中科院分区：
文献类型：
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作者：
Valles, Daniel J.;Zholdassov, Yerzhan S.;Korpanty, Joanna;Uddin, Samiha;Naeem, Yasir;Mootoo, David R.;Gianneschi, Nathan C.;Braunschweig, Adam B.
我们报告了一种新的聚糖阵列结构，其以亚飞摩尔亲合力结合甘露糖特异性聚糖结合蛋白伴刀豆球蛋白A（ConA）。 专门为此应用开发的一种新的自由基光聚合反应将硫醇-（甲基）丙烯酸酯接枝聚合与硫醇-烯化学相结合，将聚糖接枝到生长的聚合物刷上。通过使用超表面光刻法在>400种不同条件下进行这种接枝到/接枝自自由基光聚合（GTGFRP）来研究刷子的传播，超表面光刻法是一种大大加速表面上的反应发现和优化的印刷策略。刷的高度和甘露糖苷的接枝密度上的ConA的绑定刷的效果进行了系统的研究，我们发现，多价和合作的绑定帐户前所未有的灵敏度的GTGFRP刷。这项研究进一步证明了超表面光刻的优点，新的化学物质可以很容易地为应用程序量身定制。
We report a novel glycan array architecture that binds the mannose‐specific glycan binding protein, concanavalin A (ConA), with sub‐femtomolar avidity. A new radical photopolymerization developed specifically for this application combines the grafted‐from thiol–(meth)acrylate polymerization with thiol–ene chemistry to graft glycans to the growing polymer brushes. The propagation of the brushes was studied by carrying out this grafted‐to/grafted‐from radical photopolymerization (GTGFRP) at >400 different conditions using hypersurface photolithography, a printing strategy that substantially accelerates reaction discovery and optimization on surfaces. The effect of brush height and the grafting density of mannosides on the binding of ConA to the brushes was studied systematically, and we found that multivalent and cooperative binding account for the unprecedented sensitivity of the GTGFRP brushes. This study further demonstrates the ease with which new chemistry can be tailored for an application as a result of the advantages of hypersurface photolithography.