Inactivation of acyclovir-sensitive and -resistant strains of herpes simplex virus type 1 in vitro by photodynamic antimicrobial chemotherapy
Inactivation of acyclovir-sensitive and -resistant strains of herpes simplex virus type 1 in vitro by photodynamic antimicrobial chemotherapy
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发表时间：
2015-05
期刊：
影响因子：
2.2
通讯作者：
Miftahul Akhyar Latief;T. Chikama;Ji-Ae Ko;Y. Kiuchi;T. Sakaguchi;A. Obana
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作者：
Miftahul Akhyar Latief;T. Chikama;Ji-Ae Ko;Y. Kiuchi;T. Sakaguchi;A. Obana
目的评价新型卟啉衍生物TONS 504和发光二极管（LED）光动力抗菌化疗（PACT）对阿昔洛韦（ACV）敏感和耐药的单纯疱疹病毒1型（HSV-1）的疗效。方法用不同浓度（0.01 ~ 10 mg/l）的TONS 504对感染病毒株的人FL细胞进行PACT，并在不同的光能量（10 ~ 30 J/cm 2）下照射，然后孵育24 h后进行分析。结果抗HSV-1抗体的免疫细胞荧光分析显示，PACT以依赖于TONS 504浓度和光能的方式清除HSV-1和ACV耐药HSV-1。在TONS 504浓度为10 mg/l和光能为10 - 30 J/cm 2时，以及在TONS 504浓度为1 mg/l和光能为20或30 J/cm 2时，两种病毒均明显完全根除。在不存在辐照的情况下使用TONS 504或在不存在TONS 504的情况下使用辐照没有明显的抗病毒作用。用4′，6-二脒基-2-苯基吲哚对细胞核进行染色，显示PACT系统没有明显的细胞毒性，这一发现得到了系统不能诱导宿主细胞释放乳酸脱氢酶的证实。结论基于TONS 504和LED的PACT系统可有效清除HSV-1和ACV耐药HSV-1，且对宿主细胞无有害影响。
Purpose To evaluate the efficacy of photodynamic antimicrobial chemotherapy (PACT) with the new porphyrin derivative TONS 504 and a light-emitting diode (LED) against acyclovir (ACV)-sensitive and -resistant herpes simplex virus type 1 (HSV-1). Methods Human FL cells infected with the viral strains were subjected to PACT with TONS 504 at various concentrations (0.01 to 10 mg/l) and irradiation at various light energies (10 to 30 J/cm2) and were then incubated for 24 h before analysis. Results Immunocytofluorescence analysis with antibodies to HSV-1 revealed that PACT eliminated HSV-1 and ACV-resistant HSV-1 in a manner dependent on the TONS 504 concentration and light energy. Complete eradication of both viruses was apparent at a TONS 504 concentration of 10 mg/l and light energy of 10 to 30 J/cm2 as well as at a TONS 504 concentration of 1 mg/l and light energy of 20 or 30 J/cm2. No antiviral effect was apparent with TONS 504 in the absence of irradiation or with irradiation in the absence of TONS 504. Staining of cell nuclei with 4′, 6-diamidino-2-phenylindole revealed no apparent cytotoxicity of the PACT system, a finding that was confirmed by the system’s failure to induce the release of lactate dehydrogenase from the host cells. Conclusions We conclude that our PACT system based on TONS 504 and an LED is effective for eliminating HSV-1 and ACV-resistant HSV-1 without a harmful effect on host cells.