Highly Sensitive Naphthalimide-Based Fluorescence Polarization Probe for Detecting Cancer Cells
Highly Sensitive Naphthalimide-Based Fluorescence Polarization Probe for Detecting Cancer Cells
复制标题
用于检测癌细胞的高灵敏度萘酰亚胺荧光偏振探针
DOI：
10.1021/acsami.5b02429
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发表时间：
2015-05-13
影响因子：
9.5
通讯作者：
Jia, Nengqin
中科院分区：
文献类型：
--
作者：
Jia, Ti;Fu, Congying;Jia, Nengqin
基于荧光偏振（FP）的信号是一种自参考荧光信号，并且它对染料浓度和环境干扰的依赖性较小，这使得FP测量成为基于荧光强度检测的有吸引力的替代传感技术。然而，大多数的荧光偏振探针是通过引入荧光素、罗丹明和菁染料构建的，与BODIPY和萘酰亚胺染料相比，它们具有相对较短的激发态寿命。本文中，设计并合成了第一种基于萘二甲酰亚胺的荧光偏振探针（BIO），用于选择性和直接检测癌细胞。萘酰亚胺相对较长的激发态寿命和较高的光稳定性使得BIO在均匀溶液中定量测定HeLa细胞时更加灵敏和准确，而无需细胞裂解和进一步的分离步骤。BIO对HeLa细胞的检测限约为85 cells mL（-1），线性范围为2.5 × 10（2）cells mL（-1）至1 × 10（6）cells mL（-1），响应时间不超过25 min。此外，由于萘酰亚胺相对较高的光稳定性，BIO特别适合于在连续照射下用共聚焦显微镜进行活细胞成像，MO与CD 44过表达细胞系的特异性相互作用清晰可见。重要的是，这种基于BIO的传感平台在与基于萘酰亚胺的荧光偏振探针配合使用时，为癌细胞诊断提供了直接和实时的工具。
Fluorescence polarization (FP)-based signal is a self-referencing fluorescence signal, and it is less dependent on dye concentration and environmental interferences, which makes FP measurement an attractive alternative sensing technology to fluorescence intensity-based detection. However, most of the fluorescence polarization probes were constructed by introducing fluorescein, rhodamine) and cyanine dyes, which have relatively shorter excited-state lifetimes compared with BODIPY and naphthalimide dyes. Herein, a first naphthalimide based fluorescence polarization probe (BIO) was designed and synthesized for selective and direct detection of cancer cells. The relatively longer excited-state lifetimes and high photostability of naphthalimide makes BIO more sensitive and accuracy in quantitative determination of HeLa cells in homogeneous solution without cell lysis and further separation steps. The detection limit of BIO for HeLa cells was about 85 cells mL(-1), the linear range was from 2.5 x 10(2) cells mL(-1) to 1 x 10(6) cells mL(-1) and the response time is no more than 25 min Moreover, due to the relatively high photostability of naphthalimide, BIO was particularly suitable for live cell imaging under continuous irradiation with confocal microscopy, and the specific interaction of MO with CD44-overexpressing cell lines was clearly visualized. Importantly, this BIO based sensing platform offers a direct and real-time tool for cancer cell diagnosis when complemented with the use of naphthalimide-based fluorescence polarization probe.