Increased macrophage inflammatory protein-1α and -1β in BAL fluid of bronchiolitis obliterans organizing pneumonia
Increased macrophage inflammatory protein-1α and -1β in BAL fluid of bronchiolitis obliterans organizing pneumonia
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DOI：
10.1046/j.1440-1843.2003.00496.x
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发表时间：
2003-12-01
期刊：
影响因子：
6.9
通讯作者：
Sone, S
中科院分区：
文献类型：
--
作者：
Asano, T;Ogushi, F;Sone, S
目的：CC趋化因子主要对单核细胞和淋巴细胞具有趋化作用。本研究的目的是评估 CC 趋化因子、巨噬细胞炎症蛋白 (MIP)-1α 和 MIP-1β 在闭塞性细支气管炎机化性肺炎 (BOOP) 发病机制中的参与情况。 方法：通过酶联法测量从 BOOP 患者 (n = 13) 和对照患者 (CP，n = 18) 获得的支气管肺泡灌洗液 (BALF) 中 MIP-1α 和 MIP-1β 的浓度。结果：BOOP 患者 BALF 中的 MIP-1α（平均值 +/- SD；123.8 +/- 98.0 pg/mL）显着高于 CP 患者（62.5 +/- 46.1 pg/mL）。 BOOP 患者 (51.6 +/- 72.5 pg/mL) 中也检测到显着高于 CP 患者 (6.4 +/- 3.7 pg/mL) 的 MIP-1beta。 MIP-1α的浓度与BALE中的淋巴细胞百分比显着相关，MIP-1β的浓度与BALE中的淋巴细胞、中性粒细胞和嗜酸性粒细胞的数量显着相关。皮质类固醇治疗后BALF中的MIP-1α和MIP-1β均减少，并且伴随着BALE中的淋巴细胞减少结论：本研究表明，MIP-1α和MIP-1β可能在免疫炎症细胞募集到BALE中发挥重要作用。肺，可能有助于 BOOP 的发病机制。
Objective: CC chemokines are mainly chemotactic for monocytes and lymphocytes. The aim of this study was to evaluate the involvement of the CC chemokines, macrophage inflammatory protein (MIP)-1alpha and MIP-1beta, in the pathogenesis of bronchiolitis obliterans organizing pneumonia (BOOP).Methodology: The concentrations of MIP-1alpha and MIP-1beta in BAL fluid (BALF) obtained from patients with BOOP (n = 13) and control patients (CP, n = 18) were measured by enzyme-linked immunosorbent assay.Results: MIP-1alpha in BALF was significantly higher in patients with BOOP (mean +/- SD; 123.8 +/- 98.0 pg/mL) than in CP (62.5 +/- 46.1 pg/mL). Significantly higher MIP-1beta was also detected in patients with BOOP (51.6 +/- 72.5 pg/mL) than in CP (6.4 +/- 3.7 pg/mL). The concentration of MIP-1alpha significantly correlated with the percentage of lymphocytes in BALE and the concentration of MIP-1beta significantly correlated with the numbers of lymphocytes, neutrophils and eosinophils in BALE Both MIP-1alpha and MIP-1beta in BALF were decreased after corticosteroid therapy and this was accompanied by decreased lymphocytes in BALEConclusion: This study suggests that MIP-1alpha and MIP-1beta may play important roles in the recruitment of immuno-inflammatory cells into the lungs, and may contribute to the pathogenesis of BOOP.