Performance of a Surrogate SARS-CoV-2-Neutralizing Antibody Assay in Natural Infection and Vaccination Samples.
Performance of a Surrogate SARS-CoV-2-Neutralizing Antibody Assay in Natural Infection and Vaccination Samples.
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代用SARS-CoV-2中和抗体检测在自然感染和疫苗接种样本中的应用
DOI：
10.3390/diagnostics11101757
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发表时间：
2021-09-24
期刊：
影响因子：
--
通讯作者：
Hung IF
中科院分区：
文献类型：
--
作者：
Chan KH;Leung KY;Zhang RR;Liu D;Fan Y;Chen H;Yuen KY;Hung IF
严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）中和抗体（NAb）的产生是一种重要的体液反应，可以减少再感染或突破性感染。用于测量NAb生产能力水平的常规试验是活病毒中和试验。但是，这一试验必须在生物安全3级控制下进行。假病毒或替代NAb试验，如血管紧张素转换酶2抑制试验，可在2级控制下进行。本研究的目的是评价替代SARS-CoV-2 NAb检测（sNAb）与活病毒微量中和检测（vMN）的性能，该方法使用来自自然感染个体和疫苗接种者的样本。分析了从197名COVID-19患者、96名疫苗接种者和84名正常人中采集的380份血清样本。总体而言，sNAb（iFlash-2019-NAb检测试剂盒，中国深圳）的灵敏度、特异性、阳性预测值和阴性预测值分别为97.9%、94.9%、98.2%和93.8%。对于自然感染个体和疫苗接受者，测定相对于vMN的一致性分别为98.5%和93.9%。这两组sNAb和vMN之间的相关性分析得出R2值为0.83。iFlash RBD NAb检测试剂盒被发现对2019冠状病毒病患者和已接种疫苗的患者的中和抗体测量灵敏可靠。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-neutralizing antibody (NAb) production is a crucial humoral response that can reduce re-infection or breakthrough infection. The conventional test used to measure NAb production capacity levels is the live virus-neutralizing assay. However, this test must be conducted under biosafety level-3 containment. Pseudovirus or surrogate NAb tests, such as angiotensin-converting enzyme 2 inhibition tests, can be performed under level-2 containment. The aim of this study was to evaluate the performance of a surrogate SARS-CoV-2 NAb assay (sNAb) using samples from naturally infected individuals and vaccine recipients in comparison with the live virus microneutralization assay (vMN). Three hundred and eighty serum samples which were collected from 197 patients with COVID-19, 96 vaccine recipients and 84 normal individuals were analyzed. Overall, the sensitivity, specificity, positive predictive value, and negative predictive value of the sNAb (iFlash-2019-NAb assay, Shenzhen, China) were 97.9%, 94.9%, 98.2%, and 93.8%, respectively. Agreement for the assay relative to vMN for naturally infected individuals and vaccine recipients were 98.5% and 93.9%, respectively. A correlation analysis between sNAb and the vMN for both of these groups yielded an R2 value of 0.83. The iFlash RBD NAb assay is found to be sensitive and reliable for neutralizing antibody measurement in patients with the 2019 coronavirus disease and those who have been vaccinated against it.
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评估SARS-COV-2替代病毒中和试验，以检测人，犬，CAT和仓鼠血清中的抗体。
影响因子：
9.4
作者：
Perera RAPM;Ko R;Tsang OTY;Hui DSC;Kwan MYM;Brackman CJ;To EMW;Yen HL;Leung K;Cheng SMS;Chan KH;Chan KCK;Li KC;Saif L;Barrs VR;Wu JT;Sit THC;Poon LLM;Peiris M
通讯作者：
Peiris M
影响因子：
20.3
作者：
Browning SL;Gormally M;Briggs N;Li M;Zakko A;Laurent-Rolle M;Ladines-Lim J;Morrison A;Kandel P;Khan M;McLeod GX;Buller GK;Owusu KA;Treggiari MM;Hendrickson JE;Tormey CA;Desruisseaux MS
通讯作者：
Desruisseaux MS
DOI：
10.3390/v13071364
发表时间：
2021-07-14
期刊：
Viruses
影响因子：
--
作者：
Favresse J;Gillot C;Di Chiaro L;Eucher C;Elsen M;Van Eeckhoudt S;David C;Morimont L;Dogné JM;Douxfils J
通讯作者：
Douxfils J
影响因子：
56.3
作者：
To, Kelvin Kai-Wang;Tsang, Owen Tak-Yin;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
影响因子：
64.5
作者：
Hoffmann, Markus;Kleine-Weber, Hannah;Poehlmann, Stefan
通讯作者：
Poehlmann, Stefan