Release of potential immunomodulatory factors during platelet storage
Release of potential immunomodulatory factors during platelet storage
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DOI：
10.1111/j.1537-2995.2006.00869.x
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发表时间：
2006-07-01
期刊：
影响因子：
2.9
通讯作者：
Garraud, Olivier
中科院分区：
文献类型：
--
作者：
Cognasse, Fabrice;Boussoulade, Francoise;Garraud, Olivier
血小板（PLT）连接止血和炎症过程。近年来的研究表明，血小板主要通过CD 40/CD 40 L途径促进免疫和炎症反应。我们的目的是描述储存期间PLT浓缩物中细胞因子的积累。制备大量PLT浓缩物，从血浆中分离，并重悬于临床级储存培养基中;在第0、1、2、3和5天取样用于分析，不进行置换（即，不含可溶性蛋白质稀释液）。采用特异性酶联免疫吸附试验检测IL-6、IL-8、血小板源性生长因子（PDGF）-AA、可溶性CD 40配体（sCD 40 L）、RANTES和转化生长因子-β的产生。而PDGF-AA和sCD 40 L水平升高。基于先前对sCD 40 L体外诱导B细胞活化和分化的研究，在足以诱导B细胞效应的水平下定量sCD 40 L的离体产生。细胞因子和/或趋化因子水平通常较高的PLT浓缩上清液和/或PLT裂解物相比，无PLT血浆，允许确定细胞因子和/或趋化因子被吸收或分泌的输血级PLTs随时间推移，我们的数据提供的证据表明，存储的PLT含有已知的免疫调节能力的分子，并分泌他们的差异随着时间的推移，在存储过程中输血的目的。
Blood platelets (PLTs) link the processes of hemostasis and inflammation. Recent studies have demonstrated that PLTs promote immunity and inflammation mainly by means of the CD40/CD40L pathway. Our objective was to describe the accumulation of cytokines in PLT concentrates during storage.Pools of PLT concentrates were prepared, separated from plasma, and resuspended in clinical-grade storage medium; samples were taken on Days 0, 1, 2, 3, and 5 for analysis, without replacement (i.e., without soluble protein dilution). Interleukin (IL)-6, IL-8, PLT-derived growth factor (PDGF)-AA, soluble CD40 ligand (sCD40L), RANTES, and transforming growth factor-beta production were measured by specific enzyme-linked immunosorbent assays.Over time, the levels of RANTES, IL-8, and IL-6 were stable. In contrast, the levels of PDGF-AA and sCD40L increased. Ex vivo production of sCD40L was quantified at levels sufficient to induce B-cell effects based on previous studies of in vitro induced B-cell activation and differentiation by sCD40L. Cytokine and/or chemokine levels were generally higher in PLT concentrate supernatants and/or PLT lysates in comparison to PLT-free plasma, allowing the determination of which cytokine and/or chemokine was absorbed or secreted by transfusion-grade PLTs over time.Our data provide evidence that stored PLTs contain molecules with known immunomodulatory competence and secrete them differentially over time during storage for transfusion purposes.