Synaptic inputs and morphology of sustained ON-ganglion cells in the mudpuppy retina.
Synaptic inputs and morphology of sustained ON-ganglion cells in the mudpuppy retina.
复制标题
泥小狗视网膜中持续ON神经节细胞的突触输入和形态。
DOI：
10.1152/jn.1988.60.3.1143
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发表时间：
1988
影响因子：
2.5
通讯作者：
Miller,RF
中科院分区：
文献类型：
--
作者：
Arkin,MS;Miller,RF
1.持续ON-神经节细胞从mudpuppy视网膜进行了研究，与一个组合的方法，包括细胞内和细胞外记录从灌流视网膜眼杯制备，药理学浴应用2-氨基-4-膦酰基丁酸（APB），和逆行和细胞内染色辣根过氧化物酶（HRP）。2.浴应用微摩尔水平的APB选择性地阻断ON双极细胞的光响应; APB用于将突触输入分离成源自ON双极细胞与OFF双极细胞的突触输入。这种方法清楚地表明，绝大多数持续ON-神经节细胞的光响应主要是由ON-双极细胞（直接或间接）产生的持续兴奋性输入的结果。3. APB揭示了在许多持续ON神经节细胞中存在通常不明显的瞬时兴奋性OFF输入。4.五个持续ON-神经节细胞进行细胞内染色HRP和它们的形态进行了分析与计算机辅助神经元重建系统的帮助。基于对许多形态学参数的定量分析，染色的细胞在解剖学上相似。所有五种细胞的树突树主要局限于内网层的亚板层B，尽管有些细胞有少量突起在亚板层a中分叉。这些后者的过程可能涉及到的瞬态兴奋性关断输入显示与APB应用程序。5.神经节细胞的形态相似的染色，胞内持续ON-神经节细胞被发现在收集高尔基体样细胞标记的HRP逆行运输。这就提出了一种可能性，即持续在mudpuppy神经节细胞可能构成一个形态学上可识别的类视网膜神经节细胞在这个物种。也有一些建议，可能存在形态相似的OFF细胞。
1. Sustained ON-ganglion cells from the mudpuppy retina were studied with a combined approach, including intracellular and extracellular recording from the superfused retina-eyecup preparation, pharmacology with bath-applied 2-amino-4-phosphonobutyrate (APB), and retrograde and intracellular staining using horse radish peroxidase (HRP). 2. Bath application of micromolar levels of APB selectively blocks the light response of ON-bipolar cells; APB was used to separate synaptic inputs into those which originate from ON- vs. OFF-bipolar cells. This approach clearly demonstrates that the light response of the vast majority of sustained ON-ganglion cells is primarily the result of sustained excitatory inputs that arise (directly or indirectly) from ON-bipolar cells. 3. APB revealed the presence of transient excitatory OFF-inputs in many sustained ON-ganglion cells that are normally not evident. 4. Five sustained ON-ganglion cells were intracellularly stained with HRP and their morphology was analyzed with the aid of a computer-assisted neuron reconstruction system. The stained cells are anatomically similar, based on quantitative analysis of a number of morphological parameters. The dendritic trees of all five cells are primarily confined to sublamina b of the inner plexiform layer, although some cells have a small number of processes that ramify in sublamina a. These latter processes may relate to the transient excitatory OFF-inputs revealed with APB application. 5. Ganglion cells which are morphologically similar to the stained, intracellularly sustained ON-ganglion cells were found in a collection of Golgi-like cells that were labeled by retrograde HRP transport. This raises the possibility that sustained ON-ganglion cells in the mudpuppy may constitute a morphologically identifiable class of retinal ganglion cells in this species. There is also some suggestion that a morphologically similar class of OFF-cells may be present.